Introduction

As Infants and Young Children (IYC) grow, complementary foods (CFs) play a crucial role in meeting their nutritional needs when breast milk alone is no longer sufficient¹. According to Oladiran and Emmambux (2022), it is widely recognized that CF must offer sufficient energy, protein, vitamins, minerals, and other essential micronutrients to cater to the nutritional needs of children^2,3. Adequate and proper CF are crucial for the growth and cognitive development of IYC ^4,5. Recent assessments by the World Health Organization (WHO) ⁶ indicate that globally, less than 25% of infants aged 6 to 23 months adhere to the recommended dietary diversity guidelines, and only a minuscule proportion receives diets that fulfill the necessary nutritional adequacy. These findings underscore a concerning deficiency in infant nutrition at a global level.

Common CF in the East African region traditionally consists of cereal-based diets^7–10. Maize and groundnuts serve as the primary ingredients of CF but are susceptible to contamination with mycotoxins, especially aflatoxins (AFs) and fumonisins (FBs). AFs and FBs are secondary metabolites of Aspergillus ^11,12 and Fusarium ^13,14, with four types of AFs outlined by the International Agency for Research on Cancer (2002), specifically AFB₁, AFB₂, AFG₁, and AFG₂. AFB_1, the most potent variant, is responsible for approximately 75% of the overall food contamination attributed to AFs¹⁵.

The consumption of mycotoxin-contaminated foods can result in acute or chronic exposure. Chronic dietary exposure to AFs is linked to immunosuppression, liver cancer, and growth impairment. Simultaneously, ingesting higher doses can lead to acute aflatoxicosis, manifesting as severe illness and death due to damage to the liver, kidneys, and reproductive organs^16,17. FBs are associated with oesophagal cancer in adult populations and neural tube defects in infants whose mothers were exposed to high toxin levels through maize-based foods during the first trimester of pregnancy ^17–20. Despite the severe health effects and consequences of dietary exposure to AFs and FBs, mothers and caregivers in rural communities of low-income countries often lack knowledge about nutrition and mycotoxin contamination of common CF ^21–23, thereby presenting a potential health risk during complementary feeding. Recent studies by Lesuuda et al. (2021) and Ayo et al. (2018) have demonstrated a lack of awareness regarding mycotoxin contamination of foods in Kenya and Tanzania. The combined impact of traditional feeding practices and food product contamination with mycotoxins has resulted in persistent dietary exposure of IYC to AFs and FBs.

This review delves into the contamination levels of AFs and FBs in CFs consumed by infant and young children in the East African region, with a focus on assessing their potential dietary exposure to these mycotoxins. It also reviews the detection capacities for these mycotoxins to establish their strengths and weaknesses and provides an overview of practical mitigation efforts that can minimize the risks of IYC exposure to these toxins within five out of thirteen East African Countries. The information presented here contributes to understanding the magnitude of contamination in conjunction with IYC habitual diets and dietary exposure to these toxins.

Searching process

The study relies on research articles examining dietary exposure to AFs and FBs within the context of appropriate complementary feeding practices across five East African countries. This paper aims to provide a comprehensive overview of the impact of mycotoxins on children. The research involves a review of relevant literature, including scholarly manuscripts and articles that provide up-to-date insights, practical findings, and theoretical perspectives on the effects of AF and FB in the region.

Infant diets in the East African Region

This review focuses on a subset of countries in the East African region: Tanzania, Kenya, Uganda, Rwanda, and Burundi, chosen from a total of 13 countries, which includes Burundi, Comoros, Djibouti, Ethiopia, Eritrea, Kenya, Rwanda, Seychelles, Somalia, South Sudan, Sudan, Tanzania, and Uganda. The selection of these focus countries was guided by regional economic communities, as cited in the works of Nyakabwa-Atwoki (2020), Onyango et al. (2019), and Zewdie (2019). These particular countries share commonalities such as their participation in the Common Market, similarities in food production methods, and child feeding practices ^26–28. Notably, maize, which is susceptible to contamination by AFs and FBs, represents a substantial portion of their food supply ^29–31. Furthermore, these five nations also share advantageous tropical conditions that facilitate the production of mycotoxins, thereby posing significant chronic health risks (Refer to Table 1).

According to Kimanya et al. (2009), the consumption of maize by infants in rural Tanzania is relatively high. Results from this study show 89% consumed maize, ranging from 2.37 to 158 g/person/day at a mean of 43±28 g/person/day. This consumption is moderately high compared to the recommended daily maize intake as complementary food that should not exceed 20g for a child under one year of age ³⁰. In Uganda, maize is also the staple food for most of the population, including children ³¹. Moreover, it is reported that 94.4% of common foods given to children aged 6–23 months in Kitui, Kenya, consisted of maize. Another study found that the most frequently introduced first solid food to children in Wakiso District, Kenya is maize ³². Similarly, maize is commonly used in complementary feeding in Uganda ³³.

Groundnuts are legumes commonly used to enrich cereals for CF ³⁴. Reports confirm the utilization of this ingredient in Uganda ^35,36. Nassanga et al. (2018) describe groundnut flour as typical CF food in the Acoli sub-region of Uganda. In Tanzania, maize is usually pre-blended with groundnuts to a composite porridge flour locally known as lishe³⁷. In a study conducted in the Rutsiro district in Northwest Rwanda, mothers reported feeding maize thin porridge mixed with groundnut ³⁸. Another study, which assessed the types of CF and child-feeding practices in the Dodoma region, Tanzania revealed that maize, sorghum, and finger millet were the commonly used cereals in children’s diets ³⁹. Other legumes, including beans or bean soup, are given to babies as part of family meals and are occasionally used to improve the protein content of complementary porridge⁴⁰. Bean soup has also been part of CF in Ugandan babies ³². As a significant source of affordable protein and oil, soybeans have also been reported in Rwanda and Burundi⁴¹. Mashed bananas have been used in CF in a few areas, like the Kagera region of Tanzania, with slightly diverse food sources ⁴². Likewise, mashed bananas, beef soup, or sour milk are reported to be used in the Northern and Southern Highlands of Tanzania ^39,43,44. Boiled and mashed bananas have been used in Uganda and some parts of Rwanda ^38,45,46. On the other hand, Irish potatoes have been mentioned in all these five countries ^38,47–50.

Fruits, green vegetables, and animal food products are limited, as mentioned ⁵¹. Similarly, there has been minimal consumption of porridge made from sorghum and millet. ^52–55. In general, sole or multiple cereal-based, sometimes enriched with legumes, nuts, or oily seeds such as groundnuts, are the leading CF across the East African region. While some parts of these countries have access to relatively diverse foods, a majority rely on traditional staple cereals in which maize and groundnut account for the most significant proportion. However, these cereals are susceptible to AFs and FBs.

The presence of AFs and FBs in commonly complementary foods within East Africa

Despite the known health effects of mycotoxins, reports on their contamination of food crops and the subsequent exposure of IYC are relatively limited in East and sub-Saharan Africa (Table 2). Some studies specifically sampled CF are reported here, While others focused on the primary ingredients used in CF, which are likely to be fed to children. For instance, Kamala et al’s. (2016) assessment of maize samples from the northern highlands, south-western highlands, and eastern lowland zones of Tanzania, such as Kilosa, Hanang, and Rungwe revealed that 45% and 85% of the samples were contaminated with AFs and FBs, respectively, with 33% co-occurrence of both toxins. The contamination of CF and their ingredients tends to increase during storage ^56–58. For instance, in the Kongwa district of central Tanzania, an increase in mean levels of AFs was reported in stored maize from 13.12 µg/kg on day one to 14.75 µg/kg and 19.39 µg/kg at day 90 and 180, respectively ⁵⁸.

TLC = Thin Layer Chromatography, ELISA = Enzyme-linked Immunosorbent Assays, HPLC = High-Performance Liquid Chromatography

A recent study assessed total AFs in stored maize, maize flour, and stiff porridge consumed in the Dodoma Region. Samples of corn (n = 52), corn flour (n = 50), and stiff porridge (n = 34) from various schools in the Dodoma Region revealed that AFs could be found in 69.2%, 72%, and 29.4% of the samples of preserved corn, corn flour, and stiff porridge, respectively, at concentrations of 1.6-86.6 3 g/kg, 1.5-60.1 3 g/kg, and 1.8-55.1 3 g/kg ⁵⁹. Furthermore, 17 of the 52 maize samples showed AF concentrations above the maximum tolerated limit (MTL) of 10 g/kg, and around 69% of the samples had overall AF contamination.

The contamination of CF and their ingredients with AFs in Uganda is lower than in Tanzania. Research on FA levels in the market and household maize, common ingredients in CF in Kampala, established that 20% of the maize from six markets was contaminated with AFs ranging from 0.7 to 88.6 μg/kg with a mean contamination level of 7.6 ± 2.3 µg/kg ⁶⁰. The same study in Uganda revealed widespread AF contamination in household samples, with 74% containing AFs, with a mean level of 22.2 ± 4.6 µg/kg. Notably, one sample reached a staggering 268 µg/kg, exceeding the East African region limit of 10 µg/kg by over 26 times, although this contaminated ingredient was not specifically intended for CF. Furthermore, the occurrence of FBs appears heavily influenced by altitude, favouring Fusarium growth in high-altitude areas. A study comparing FB contamination in maize samples across Uganda three agroecological zones (high attitude, mid-altitude moist, and mid-altitude dry) underscored this trend. High-altitude areas exhibited significantly higher FB levels, ranging from a concerning 850 to 10,000 µg/kg with an average of 4,930 µg/kg, which is more than double the EAC limit of 2,000 µg/kg. In contrast, the mid and high altitudes showed substantially lower concentrations, ranging from 0.47 to 8.3 and 0.85 to 10 µg/kg, respectively ⁶¹.

A study to ascertain the level of AF contamination in peanut butter and groundnuts in Nairobi and Nyanza, Kenya involved obtaining eighty-two samples comprising raw and roasted groundnuts and peanut butter from the market of these two areas. AF levels in all samples ranged from 0 to 2377.1 μg/kg ⁶². Another study by Menza et al. (2015), which assessed the contamination of groundnuts with AFs in Busia and Kisii Central districts in western Kenya revealed that 97.06% of the groundnut samples from Busia were contaminated with AFs in the range of 0.1 to 268 µg /kg, while all groundnut samples from Kisii were contaminated with AFs in the range of 1.63 to 591.1 µg/kg. Although groundnuts are not CF, they are commonly used in East African countries as CF ingredients, and thus, they are likely to be consumed by infants and exposed to AFs.

Mirroring the situation in Uganda, maize samples from Rwandan markets, often used as ingredients, exhibit concerning levels of AF contamination. A study quantifying AFB1 at two-time points in 684 maize flour samples in Kigali retail markets, intended for both family and CF, revealed substantial variations. In some markets, mean levels reached a concerning 24.7 + 23.7 µg/kg, with a maximum of 98.6 µg/kg. Conversely, other markets displayed lower mean levels of 8.0 + 5.6 µg/kg. A second analysis confirmed this trend, with mean levels ranging from 10.2 ± 8.4 µg/kg to 25.7 + 25.9 µg/kg and a maximum of 116.9 µg/kg ^63,64. Further investigation into CF ingredients ((maize, groundnut, and cassava flour), across four Rwandan provinces revealed widespread mycotoxin contamination. Maize, a key ingredient, showed contamination in 89 of the samples, with AFs and FBs reaching 16.8 µg/kg and 48.1 µg/kg, respectively. Groundnut samples, another potential ingredient, exhibited a 100% contamination rate with AFs reaching 126.6 µg/kg. Notably, cassava flowers, while less prone to contamination, still showed AFs in 33% of samples, with concentrations reaching 2.7 µg/kg, while FBs were either non-detectable or present at low levels ⁶⁵.

Burundi, like other East African nations, exhibits a widespread presence of AFs and FBs in CF ingredients. A May – July 2016 market survey of 244 sorghum, maize, and groundnuts samples revealed that 218 (88.5%) were contaminated with AFs, ranging from 1.3 to 2,410 µg/kg levels and an average of 117 µg/kg ⁶⁸. Sorghum displayed the highest range (2.5-490 µg/kg), followed by maize (2.5-350 µg/kg) and groundnuts (2.2-2,410 µg/kg). Sorghum displayed the highest range (2.5 and 490 µg/kg), followed by maize (2.5 to 350 µg/kg), and grounds (2.2 to 2,410 µg/kg). Notably, these concentrations significantly exceed those documented in Tanzania, specifically in the Rombo district. There, maize and maize-based CF contained AFs in the range of 0.11 to 386 µg/kg. ⁶⁷. Consistent with other East African countries, FBs were also prevalent in Burundian CF ingredients, particularly in maize (15-2,918 µg/kg) and sorghum (3-374 µg/kg) ⁶⁶. This widespread contamination of CF with AFs and FBs across the region, as evidenced by the reviewed literature, is likely driven by various factors, as discussed in this subsection ⁶⁸.

Factors promoting AFs and FBs contamination of common CF within the EAC

Numerous factors contribute to an elevated risk of AFs and FBs in the East African regions, encompassing climate variations, recurrent droughts, pest invasions, inappropriate farming and storage methods, socioeconomic status, limited awareness, detection methodologies, deficient regulatory oversight, and persistent food insecurity. Many food commodities in the EAC, including those used as CF, can be contaminated with mycotoxins at pre- or post-harvest ^69–73. Food contamination with mycotoxins in the EAC is primarily exacerbated by the tropical and sub-tropical climates that strongly favour the growth of A. flavus. For example, regions situated within the latitudinal range of 40ºN to 40ºS, including East Africa, are conducive to mold proliferation and elevate the potential for AF exposure ^74. This phenomenon occurs because elevated temperatures support the production of A. flavus conidia, their dispersion, and an increased kernel infection rate, all collectively contributing to the accumulation of AFs in various food products ⁷⁵. This predominantly occurs in lower elevations, which typically experience higher temperatures and humidity, in contrast to higher altitudes characterized by lower temperatures and humidity levels ⁷³.

Delayed harvesting is a common practice in different parts of the East Africa region that further exposes crops to fungal growth and contamination with AFs and FBs  ⁷⁶. A previous study by Kaaya et al. ⁷⁷ recorded a seven-time increase in AFs when maize harvesting was delayed for a month in Uganda. The duration of storage can also influence the contamination of primary ingredients of CF with AFs and FBs. For instance, a substantial correlation was established between the quantities of AFs detected in stored maize following extended periods in agroecological regions characterized by dry climates. ⁷⁸. AFs can be generated within agricultural commodities intended for complementary feeding 79,80 during storage. In Dodoma, Tanzania, Aron et al. and Ngoma et al. showed that various post-harvest practices significantly associated with AFs in complimentary composite flours included storage of cereals for over 12 months. Besides, numerous small-scale farmers frequently subject maize and other cereal grains to suboptimal storage conditions for prolonged periods before their utilization or sale, thus inadvertently fostering an environment conducive to fungal proliferation and AF contamination ^76,79. Again, when crops are not appropriately dried or kept in poorly ventilated stores with high relative humidity, as has been established in peanuts in markets and farms in Uganda ⁷⁷. According to Villers ⁸¹, aflatoxigenic fungi thrive when the relative humidity of food commodities in storage surpasses the critical level that supports fungal growth. This is because temperature and water activity significantly affect Aspergillus growth and gene expression for the biosynthesis of AFs ⁸².

On occasion, cereals are gathered with increased moisture levels and subsequently dried to lower their moisture content prior to storage. This delayed drying process elevates the potential for mold growth and the formation of AFs and FBs. Similarly, most farmers are reported not to be sorting or dehulling cereals like maize meant for infant feeding, which increases the risk of contamination with mycotoxins like AFs and FBs. Ngoma et al., for instance, established that a majority (81.3%) of 364 study respondents in some districts in central Tanzania needed to be dehulling the crops used in preparing CF for children aged between 6 and 23 months. Makori et al. (2019) say this is a common contributor to the risk of contamination and exposure to AFs.

Several foodstuffs that constitute primary ingredients for CF are often dried on bare ground, predisposing them to contamination with AFs and FBs. The study by  ^76,79 in the central parts of Tanzania also reported a positive association between drying of maize on bare ground and mycotoxin contamination, which was accelerated when harvested maize was dried without husks because then the maize grains are in direct contact with soil, which is a source of molds. Substandard storage practices are also often associated with the contamination of common ingredients of CF.

The contamination of CF with AFs after harvest is possible under conditions that promote the growth of aflatoxigenic fungi during various stages, including harvesting, storage, milling, and distribution at both the market and household levels ⁸³. Aspergillus flavus exhibits relatively higher moisture needs compared to other types of fungi. Consequently, the risk of grain contamination increases when the seed moisture content is elevated. For example, the presence of AFs in harvested maize is affected by elevated temperatures and moisture levels, typically ranging from 12 to 40°C and 3-18%, respectively ⁸⁴. Building upon Coppock et al.’s findings, a confluence of factors can exacerbate aflatoxin and fumonisin contamination in East African crops. Delayed harvests due to wet conditions, combined with sufficient heat for aflatoxigenic fungi growth, create a perfect storm for toxin production and contamination. Additionally, any damage incurred by kernels or nuts during harvesting, cleaning, and handling processes can compromise their integrity, thereby creating entry points for fungal invasion and subsequent contamination ⁸³. However, it is crucial to recognise that inadequate household handling and storage facilities often emerge as even more potent factors, warranting special attention. Section 5 discusses analytical techniques for detecting AFs and FBs in food in the East African region. However, despite the prevalent mycotoxin contamination of essential crops in this area, the ability to conduct thorough analyses faces limitations due to inadequate infrastructure, skillsets, and the absence of rapid detection tools capable of onsite toxin identification before the harvest, sale, purchase, or consumption of vulnerable crops.

Analytical methods for AFs and FBs in foods in the EAC

High-Performance Liquid Chromatograph (HPLC) and Enzyme-Linked Immunosorbent Assay (ELISA) are the standard analytical methods used for detecting and quantifying AFs and FBs in foods, including CF in the East African region. For instance,  ⁷² recently used ELISA  to quantify AFs in sorghum, millet, finger millet, groundnuts, composite flour (lishe), and FBs in maize, with comparable results. Kibwana et al. also used ELISA to assess the levels of AFs in stored maize, maize flours, and stiff porridge in schools in Dodoma, Tanzania. Clavel and Brabet (2013) assessed groundnut samples from Busia and Homabay districts in Kenya using ELISA and established levels of AFs ranging from 0 – 2688 µg/kg and 0-7525 µg/kg, respectively. According to Mollay et al., HPLC was used to detect AFs in maize, sorghum, pearl millet, rice, and groundnuts in the samples collected in the Kongwa district of Dodoma region. Similarly, Makori et al. (2019) used HPLC to analyze AF in CF samples collected in Chamwino Dodoma.

Despite its established efficacy in detecting food aflatoxins (AFs), Enzyme-Linked Immunosorbent Assay (ELISA) faces inherent limitations for field-based applications. While popular among scientists³ for its reliability and simplicity in sample preparation, ELISA’s dependence on laboratory settings and highly skilled personnel renders it impractical for field testing. Due to its popularity, attempts have been made to improve the sensitivity of ELISA for detecting mycotoxins ⁶. Recognising this, research has shifted towards integrating specific molecular recognition elements, like immunochemical, directly with portable transduction systems to facilitate rapid, non-laboratory mycotoxin detection. HPLC, on the other hand, serves as a robust and globally accepted quantitative method for food analysis and mycotoxins detection. Its impressive sensitivity and versatility, allowing simultaneous detection of over 50 analytes at µg/kg levels, solidify its position as the industry standard for mycotoxin testing ^88,89. The integration of the Immunoaffinity column (IAC) into HPLC further enhances its effectiveness by minimizing its mycotoxin loss and eliminating interfering substances through specific antigen-antibody interactions ⁹⁰. This combined approach has proven particularly accurate and valuable for evaluating AFs in CF, enabling informed strategy development and status assessments^91,92. However, HPLC’s advantages come at a cost. The technique’s demanding sample preparation steps, requiring laboratory supplies and organic solvents, can be laborious and expensive. Consequently, its application is limited to small sample sizes and well-funded studies.

These methods were reviewed to present how the toxins are quantified in different countries for regional comparison. The suitability of a method depends on its safety, effectiveness, ease of use, affordability, speed, and ruggedness. Although progress has been made in the region toward detecting AFs and FBs in ingredients used in CF, more efforts are needed to enhance the mycotoxin analysis specifically for CF and infant diets at all levels. Also, common methods have been discussed to connect with regulations enforcing food safety within the region. Mycotoxin regulation is necessary to reduce food contamination ⁹³. (Table 4), Implementing regulations in low-resource settings with pronounced food insecurity, limited testing technologies, and facilities can be challenging.

Documentation of IYC exposure to AFs and FBs in the East African region

Numerous research studies have illustrated the persistent dietary exposure of IYC infants and in EAC mycotoxins over extended periods. For instance, investigators have documented elevated AF exposure levels in Tanzania attributable to diets heavily reliant on maize-based foods ^92–94. In Rombo district located in Northern Tanzania, the estimated dietary intake of AFs and FBs in 143 infants was found to be 0.14 to 120 ng kg^-1 body weight (bw) per day and 0.005 to 0.88 μgkg⁻¹ bw per day, respectively ⁹⁵. Infants and young children are considered particularly susceptible to exposure to AFs and FBs due to their reduced body weight and accelerated metabolic rate ⁹⁶. Children in the East African region are exposed to AFs and FBs mainly through CF (Table 3). While IYC can be exposed to AFs through various means, including in utero transmission from the mother through the placenta ⁹⁷, breastfeeding ⁹⁸, or direct ingestion of contaminated foods ^99,100, it is essential to note that CF remains the primary source of AFs and FBs exposure among IYC. An upsurge in harm to children’s health, particularly their immune and digestive systems, has been linked to staple CFs contaminated with AFs. It impairs their physical development, causing stunting, wasting, and underweight conditions. At the same time, it hinders their learning and development. It is crucial to grasp the extent of exposure, its impact, and methods for diminishing IYC’s susceptibility to AFs and FBs in the region.

Makori et al. conducted a study in the Dodoma region of Tanzania, where they examined CF and exposure to AFB₁ among a group of 101 children aged 6-23 months. Their investigation unveiled a broad range of exposure levels from 0.1 to 23,172.81 ng/kg bw/day. Another study on 18 to 24-month-old children in Rombo District, Tanzania, reported exposure to AFs at 1 to 786 μg/kg bw/day and FBs at 0.38 to  8.87 μg/kg bw/day ⁶⁷. Moreover, a research investigation conducted in Kongwa, Tanzania, examined the exposure risk among children aged 6 to 12 months and documented a range of AFB₁ exposure levels from 0.33 to 1168 ng/kg bw/day, with a median value of 23.08 ng/kg bw/day ¹⁰¹.

On the other hand, a study done in Kisumu, Kenya, reported that the AF exposure of children aged 4 -6 months ranged from 4.43 to 110.4 ng/kg bw/day from the consumption of CF, including groundnuts, cassava, maize, and sorghum ¹⁰². Another cross-section study in two low-income areas of Kenya revealed that 98% of the food samples collected tested positive for AFs, resulting in an average exposure of 21.3 µg/kg bw/day ¹⁰³. Likewise, one of the cohort studies in Mukono district, Uganda, found an association between maternal AFs exposure of median maternal AFB-Lys level 5.83 pg/mg albumin (range: 0.71–95.60 pg/mg albumin, interquartile range: 3.53–9.62 pg/mg albumin during pregnancy and adverse birth outcomes. The study reported adverse birth outcomes, notably lower birth weight and smaller head circumference ¹⁰⁴. In EAC, Tanzania reported high AF exposure compared to other EAC countries, although most said AF exposure exceeded the exposure of concern of 0.017 ng/kg bw/day ⁶⁷. Even with this wide exposure range, enforcing mycotoxins regulation in these countries is still challenging.

Mycotoxin regulation and mitigation strategies in the East African region

In the East African region, the vulnerability of infants to AFs and FBs through the consumption of contaminated maize remains a pressing concern, despite the established regulations. This issue is exacerbated by the decentralized nature of small-scale maize farming, where regulatory bodies must navigate the challenges presented by widely dispersed farmers who produce and distribute maize to the broader population ¹⁰⁵. The regulatory mechanisms have also focused on post-harvest interventions and rarely on pre-harvest and harvesting conditions. However, such field practices and technologies can also be regulated to minimize the fungal colonization of crops ^106,107. Moreover, discrepancies in implementing regulations have been reported. For instance, in the EAC, no specific standards are set for infant diets, which increases the risks of child exposure to mycotoxins and their subsequent health effects ¹⁰⁸. Owing to the immense health impacts of AFs and FBs on IYC, stringent regulations for food intended for IYC and additional mitigation strategies should be implemented to reduce dietary exposure to this vulnerable group.

Even though standards exist for food and food products for the general population, inspection for mycotoxin contamination is mainly performed on food products aimed at the export market. In contrast, locally traded foods, especially those meant for use as CF, are often not tested ¹⁰⁹. Unless awareness creation and demand for local clean food are highly prioritized among consumers, implementing the regulation for local foods will take a long time to realize. Given that food can be contaminated in the field during harvest, transportation, marketing, and storage ¹¹⁰ customized strategies to mitigate mycotoxin contamination throughout the value chain support adherence to the regulations. These strategies include hand sorting (before storage and use), drying maize on mat/raised platforms, proper sun drying, applying storage insecticides, and dehulling before milling ⁷. The application of sorting as a common strategy in reducing fungal growth and toxin production in food products has also been documented in Burundi ⁶⁶. Other studies have shown that sorting maize may not necessarily reduce AFs but can significantly reduce FBs ^111,112. Some low-cost grain sorting technologies have recently been developed to reduce mycotoxin contamination in maize and groundnut ¹¹². All these have been observed to be weak in these countries, and suggestions to control the moisture, temperature, and humidity in harvested and stored grains are also a core mitigation strategy to reduce fungal growth and grain contamination have been made ⁵⁷.

Enhancing skills through training in suitable pre- and post-harvest practices has been identified as a viable approach for mitigating AFs and FBs at the household level ¹¹¹. Additionally, in a cluster randomized controlled trial conducted by Kamala et al., it was demonstrated that manual sorting and dehulling led to a substantial reduction in AFs and FBs levels in maize, subsequently lowering the dietary exposure of Tanzanian infants. The same research also found that drying maize on elevated platforms, plastic sheets, or canvas surfaces effectively reduced AFs compared to drying maize directly on bare ground. Another mitigation study after the 2004 outbreak of AFs in Kenya, where the Ministry of Public Health and Sanitation focused on preventive strategies to reduce dietary contamination of AFs in homegrown maize by changing the standard limits for total AFs from 20 to 10 µg/kg ¹¹³. However, lowering acceptable limits without stringent measures to ensure clean food production and monitoring locally consumed foods remains challenging.

Generally, widespread poverty and hunger in the East African region are some of the barriers reported to hinder the enforcement of mycotoxin regulation and mitigation efforts ^114,115. The regulation and mitigation strategies for mycotoxins in the East African region are insufficient, and those in place are still inadequately implemented. Therefore, intensified efforts, including Hazard Analysis Critical Control Point (HACCP) in the production process and land preparation crop rotation, planting and intercropping, and the application of botanical extracts and fungal biocontrol agents considered the most critical pre-harvest practices for controlling mycotoxin production are urgent and necessary in this regard¹¹⁶.

Conclusions and future direction

Compelling evidence from this review illuminates the widespread dietary contamination and exposure of IYC to AFs and FBs in the East African region. Despite notable advancements in mycotoxin research and the rollout of mitigation interventions, tangible evidence of a decline in this public health threat remains elusive. A multifaceted approach that integrates robust regulatory enforcement, community engagement, and frequent monitoring appears crucial in achieving this goal. This includes targeted interventions focused on improving food handling practices throughout the food chain, paired with frequent monitoring and awareness campaigns to cultivate a consistent demand for clean food within the community. This combined approach can effectively help reduce the problem. Similarly, enforcing mitigation strategies encompassing adopting correct pre- and post-harvest practices and appropriate handling of foods at the household level, can significantly contribute to addressing the issue. This holistic approach aims to reduce contamination and limit the exposure of IYC to AFs and FBs via CF in resource-constrained settings across the East African region.

Acknowledgment

The author acknowledges funding support from the Bill and Melinda Gates Foundation through the Mycotoxin Mitigation Trial (MMT), grant number OPP361155626 Authors acknowledge other MMT project investigators; Prof. Erica Phillips  of University of Wisconsin – Madison,  Prof. Paul C. Turner of the School of Public Health, University of Maryland, USA, and Prof. Rebecca Stoltzfus of Goshen College, Indiana USA and Division of Nutritional Sciences, Cornell University, USA for their involvement in framing the focus of this manuscript.

Funding Sources

There is no funding sources.

Conflict of Interest

The authors declare that there is no conflict of interest.

Authors Contribution

RAK; participated in the framing of the idea, performed literature search and review, and drafted the manuscript. NK and FN conceptualized and framed the focus of the manuscript, and critically reviewed multiple versions of the manuscript. All authors reviewed the results and approved the final version of the manuscript.

Data Availability Statement

Not Applicable

Ethics Approval Statement

Not Applicable

References

1. Dewey KG. The challenge of meeting nutrient needs of infants and young children during the period of complementary feeding: an evolutionary perspective. The Journal of Nutrition. 2013;143(12):2050-2054.
   CrossRef
2. Dewey KG. Nutrition, growth, and complementary feeding of the breastfed infant. Pediatric Clinics of North America. 2001;48(1):87-104.
   CrossRef
3. Dewey KG, Brown KH. Update on technical issues concerning complementary feeding of young children in developing countries and implications for intervention programs. Food and nutrition bulletin. 2003;24(1):5-28.
   CrossRef
4. Ijarotimi SO, Keshinro OO. Determination of nutrient composition and protein quality of potential complementary foods formulated from the combination of fermented popcorn, African locust and bambara groundnut seed flour. Polish Journal of Food and Nutrition Sciences. 2013;63(3).
   CrossRef
5. Ogunlade I, Ilugbiyin A, Ajayi IO. A comparative study of proximate composition, antinutrient composition and functional properties of Pachira glabra and Afzelia africana seed flours. A comparative study of proximate composition, antinutrient composition and functional properties of Pachira glabra and Afzelia africana seed flours. 2011;5(1):1-4.
6. Kamala A, Kimanya M, De Meulenaer B, et al. Post-harvest interventions decrease aflatoxin and fumonisin contamination in maize and subsequent dietary exposure in Tanzanian infants: a cluster randomised-controlled trial. World Mycotoxin Journal. 2018;11(3):447-458.
   CrossRef
7. Kimanya ME, De Meulenaer B, Tiisekwa B, et al. Co-occurrence of fumonisins with aflatoxins in home-stored maize for human consumption in rural villages of Tanzania. Food additives and contaminants. 2008;25(11):1353-1364.
   CrossRef
8. Kinabo JL, Mwanri AW, Mamiro PS, et al. Infant and young child feeding practices on Unguja Island in Zanzibar, Tanzania: a ProPAN based analysis. Tanzania Journal of Health Research. 2017;19(3).
   CrossRef
9. Kulwa KB, Kinabo JL, Modest B. Constraints on good child-care practices and nutritional status in urban Dar-es-Salaam, Tanzania. Food and Nutrition Bulletin. 2006;27(3):236-244.
   CrossRef
10. Egal S, Hounsa A, Gong Y, et al. Dietary exposure to aflatoxin from maize and groundnut in young children from Benin and Togo, West Africa. International Journal of Food Microbiology. 2005;104(2):215-224.
    CrossRef
11. Groopman JD, Kensler TW, Wild CP. Protective interventions to prevent aflatoxin-induced carcinogenesis in developing countries. Annu Rev Public Health. 2008;29:187-203.
    CrossRef
12. Anfossi L, Baggiani C, Giovannoli C, Giraudi G. Occurrence of aflatoxin M1 in dairy products. Aflatoxins detection, measurement and control. Published online 2011.
    CrossRef
13. Fandohan P, Hell K, Marasas W, Wingfield M. Infection of maize by Fusarium species and contamination with fumonisin in Africa. African Journal of Biotechnology. 2003;2(12):570-579.
    CrossRef
14. Domijan AM. Fumonisin B1: A neurotoxic mycotoxin. Arhiv za higijenu rada i toksikologiju. 2012;63(4):531-543.
    CrossRef
15. Pribil AI. Perils of the Fungal Kingdom: Mycotoxins in Food and Feed. Published online 2018.
16. Shephard GS. Risk assessment of aflatoxins in food in Africa. Food Additives and Contaminants. 2008;25(10):1246-1256.
    CrossRef
17. Alizadeh AM, Roshandel G, Roudbarmohammadi S, et al. Fumonisin B1 contamination of cereals and risk of esophageal cancer in a high-risk area in northeastern Iran. Asian Pacific journal of cancer prevention. 2012;13(6):2625-2628.
    CrossRef
18. Mutiga SK, Hoffmann V, Harvey JW, Milgroom MG, Nelson RJ. Assessment of aflatoxin and fumonisin contamination of maize in western Kenya. Phytopathology. 2015;105(9):1250-1261.
    CrossRef
19. Van der Westhuizen L, Shephard GS, Rheeder J, et al. Simple intervention method to reduce fumonisin exposure in a subsistence maize-farming community in South Africa. Food Additives and Contaminants. 2010;27(11):1582-1588.
    CrossRef
20. Geresomo NC. Improving Safety and Quality Of Complementary Foods for Children Aged 6-23 Months in Rural Areas of Malawi Through the Hazard Analysis and Critical Control Point Strategy. Egerton University; 2019.
21. Memon Y, Sheikh S, Memon A, Memon N. Feeding beliefs and practices of mothers/caregivers for their infants. J Liaquat Uni Med Health Sci. 2006;5(1):8-13.
    CrossRef
22. Seetha A, Tsusaka TW, Munthali TW, et al. How immediate and significant is the outcome of training on diversified diets, hygiene and food safety? An effort to mitigate child undernutrition in rural Malawi. Public Health Nutrition. 2018;21(6):1156-1166.
    CrossRef
23. Nyakabwa-Atwoki R. The Role of Donor Funds in Reducing Risks and Attracting Foreign Investments for Africa’s Geothermal Resource Development. In: Vol 11. ; 2020.
24. Zewdie A. Intergovernmental Authority on Development and East African Community: Viability of Merger. International Journal of African Development. 2019;5(2):7.
25. Onyango AW, Jean-Baptiste J, Samburu B, Mahlangu TLM. Regional overview on the double burden of malnutrition and examples of program and policy responses: African region. Annals of Nutrition and Metabolism. 2019;75(2):127-130.
    CrossRef
26. De Bruyn J, Bagnol B, Darnton‐Hill I, Maulaga W, Thomson PC, Alders R. Characterising infant and young child feeding practices and the consumption of poultry products in rural Tanzania: A mixed methods approach. Maternal & child nutrition. 2018;14(2):e12550.
    CrossRef
27. Mollay C, Kassim N, Stoltzfus R, Kimanya M. Complementary feeding in Kongwa, Tanzania: Findings to inform a mycotoxin mitigation trial. Maternal & Child Nutrition. 2021;17(4):e13188.
    CrossRef
28. Muhimbula HS, Issa-Zacharia A, Kinabo J. Formulation and sensory evaluation of complementary foods from local, cheap and readily available cereals and legumes in Iringa, Tanzania. African Journal of Food Science. 2011;5(1):26-31.
29. Abeshu MA, Lelisa A, Geleta B. Complementary feeding: review of recommendations, feeding practices, and adequacy of homemade complementary food preparations in developing countries–lessons from Ethiopia. Frontiers in nutrition. 2016;3:41.
    CrossRef
30. Kulwa KB, Mamiro PS, Kimanya ME, Mziray R, Kolsteren PW. Feeding practices and nutrient content of complementary meals in rural central Tanzania: implications for dietary adequacy and nutritional status. BMC Pediatrics. 2015;15:1-11.
    CrossRef
31. Mamiro PS, Kolsteren P, Roberfroid D, Tatala S, Opsomer AS, Van Camp JH. Feeding practices and factors contributing to wasting, stunting, and iron-deficiency anaemia among 3- to 23-month-old children in Kilosa district, rural Tanzania. Journal of Health, Population and Nutrition. Published online 2005:222-230.
32. Kimanya ME, Shirima CP, Magoha H, et al. Co-exposures of aflatoxins with deoxynivalenol and fumonisins from maize-based complementary foods in Rombo, Northern Tanzania. Food Control. 2014;41:76-81.
    CrossRef
33. Haggblade S, Dewina R. Staple Food Prices in Uganda.; 2010.
34. Ssemukasa EL, Kearney J. Complementary feeding practices in Wakiso district of Uganda. African Journal of Food, Agriculture, Nutrition and Development. 2014;14(4):9085-9103.
    CrossRef
35. Grosshagauer S, Milani P, Kraemer K, et al. Inadequacy of nutrients and contaminants found in porridge‐type complementary foods in Rwanda. Maternal & Child Nutrition. 2020;16(1):e12856.
    CrossRef
36. Oyeyinka AT, Obilana AO, Siwela M. The Potential of Bambara Groundnut for Use in Complementary Feeding. In: Food and Potential Industrial Applications of Bambara Groundnut. Springer; 2021:169-187.
    CrossRef
37. Asiki G, Seeley J, Srey C, et al. A pilot study to evaluate aflatoxin exposure in a rural Ugandan population. Tropical Medicine & International Health. 2014;19(5):592-599.
    CrossRef
38. Atukwase A, Kaaya AN, Muyanja C. Dynamics of Fusarium and fumonisins in maize during storage–a case of the traditional storage structures commonly used in Uganda. Food control. 2012;26(1):200-205.
    CrossRef
39. Mosha T, Laswai H, Tetens I. Nutritional composition and micronutrient status of homemade and commercial weaning foods consumed in Tanzania. Plant Foods for Human Nutrition. 2000;55:185-205.
    CrossRef
40. Dusingizimana T, Weber JL, Ramilan T, Iversen PO, Brough L. A qualitative analysis of infant and young child feeding practices in rural Rwanda. Public Health Nutrition. 2021;24(12):3592-3601.
    CrossRef
41. Lyimo M, Muzanila Y. Assessment of complementary foods and child feeding practices in Dodoma Region, Tanzania. Published online 2014.
42. Ahishakiye J, Bouwman L, Brouwer ID, Matsiko E, Armar-Klemesu M, Koelen M. Challenges and responses to infant and young child feeding in rural Rwanda: a qualitative study. Journal of Health, Population and Nutrition. 2019;38(1):1-10.
    CrossRef
43. Niyibituronsa M, Onyango A, Gaidashova S, et al. Evaluation of mycotoxin content in soybean (Glycine max l.) grown in Rwanda. African Journal of Food, Agriculture, Nutrition and Development. 2018;18(3):13808-13824.
    CrossRef
44. Mbela DEN. Modified Diets to Improve Iron, Vitamin A and Protein Intake among Children in Banana-Based Farming Systems of Kagera Region, Tanzania. Sokoine University of Agriculture; 2018.
45. Mgongo M, Hussein TH, Stray-Pedersen B, Vangen S, Msuya SE, Wandel M. “We give water or porridge, but we don’t really know what the child wants:” A qualitative study on women’s perceptions and practises regarding exclusive breastfeeding in Kilimanjaro region, Tanzania. BMC pregnancy and childbirth. 2018;18(1):1-9.
    CrossRef
46. Tesha AP. Improving Nutrient Content of the Frequently Used Complementary Foods for Children Aged 6-23 Months in Rombo District, Kilimanjaro. Sokoine University of Agriculture; 2017.
47. Ekesa B, Nabuuma D, Kennedy G. Content of iron and vitamin A in common foods given to children 12–59 months old from north Western Tanzania and central Uganda. Nutrients. 2019;11(3):484.
    CrossRef
48. Umugwaneza M, Havemann-Nel L, Vorster HH, Wentzel-Viljoen E. Factors influencing complementary feeding practices in rural and semi-urban Rwanda: a qualitative study. Journal of Nutritional Science. 2021;10.
    CrossRef
49. Custodio E, Herrador Z, Nkunzimana T, Węziak-Białowolska D, Perez-Hoyos A, Kayitakire F. Children’s dietary diversity and related factors in Rwanda and Burundi: A multilevel analysis using 2010 Demographic and Health Surveys. PLoS One. 2019;14(10):e0223237.
    CrossRef
50. Nankumbi J, Muliira JK. Barriers to infant and child-feeding practices: a qualitative study of primary caregivers in rural Uganda. Journal of health, population, and nutrition. 2015;33(1):106.
51. Raymond J, Kassim N, Rose JW, Agaba M. Optimal formulations of local foods to achieve nutritional adequacy for 6–23-month-old rural Tanzanian children. Food & Nutrition Research. Published online 2017.
    CrossRef
52. Thuita FM, Pelto GH, Musinguzi E, Armar‐Klemesu M. Is there a “complementary feeding cultural core” in rural Kenya? Results from ethnographic research in five counties. Maternal & Child Nutrition. 2019;15(1):e12671.
    CrossRef
53. Okoth SA, Ohingo M. Dietary aflatoxin exposure and impaired growth in young children from Kisumu District, Kenya: a cross-sectional study. African journal of health sciences. 2004;11(1):43-54.
    CrossRef
54. Mollay C, Kimanya M, Kassim N, Stoltzfus R. Main complementary food ingredients contributing to aflatoxin exposure to infants and young children in Kongwa, Tanzania. Food Control. 2022;135:108709.
    CrossRef
55. Ngure FM, Kassim N, Phillips EL, Turner PC. Infant and Young Child Feeding Practices and Mycotoxin Contamination of Complementary Food Ingredients in Kongwa District, Tanzania. Current Developments in Nutrition. 2023;7(2):100030.
    CrossRef
56. Thairu L. Achieving optimal infant and young child feeding practices: case studies from Tanzania and Rwanda. Infant and young child feeding: challenges to implementing a global strategy Chichester, United Kingdom: Wiley-Blackwell. Published online 2009:99-118.
57. Uwiringiyimana V, Veldkamp A, Amer S. Stunting spatial pattern in Rwanda: an examination of the demographic, socio-economic and environmental determinants. Geospatial health. 2019;14(2).
    CrossRef
58. Maina AW, Wagacha JM, Mwaura FB, Muthomi JW, Woloshuk CP. Postharvest practices of maize farmers in Kaiti District, Kenya and the impact of hermetic storage on populations of Aspergillus spp. and aflatoxin contamination. Published online 2016.
    CrossRef
59. Matrona EK, Anselm PM, Humphrey PN, Purificator AK, Gasper GS, Jamal BK. Smallholder farmers’ storage practices and awareness on aflatoxin contamination of cereals and oilseeds in Chamwino, Dodoma, Tanzania. Journal of Cereals and Oilseeds. 2022;13(1):13-23.
60. Sasamalo MM, Mugula JK, Nyangi CJ. Aflatoxins contamination of maize at harvest and during storage in Dodoma, Tanzania. International Journal of Innovative Research and Development. 2018;7(6):11-15.
61. Kibwana M, Kimbokota F, Christopher R, Mmongoyo JA. Aflatoxins in stored maize, maize flours, and stiff porridge consumed in schools: A case study of Dodoma region, Tanzania. Food Control. 2023;146:109519.
    CrossRef
62. Paul Wacoo A, Wendiro D, Nanyonga S, Hawumba JF, Sybesma W, Kort R. Feasibility of a novel on-site detection method for aflatoxin in maize flour from markets and selected households in Kampala, Uganda. Toxins. 2018;10(8):327.
    CrossRef
63. Atukwase A, Kaaya AN, Muyanja C. Factors associated with fumonisin contamination of maize in Uganda. Journal of the Science of Food and Agriculture. 2009;89(14):2393-2398.
    CrossRef
64. Ndung’u J, Makokha A, Onyango C, et al. Prevalence and potential for aflatoxin contamination in groundnuts and peanut butter from farmers and traders in Nairobi and Nyanza provinces of Kenya. Journal of Applied Biosciences. 2013;65.
    CrossRef
65. Matsiko F, Kanyange C, Ingabire G, Dusingizimana T, Vasanthakaalam H, Kimonyo A. Detection and quantification of aflatoxin in cassava and maize flour sold in Kigali open markets, Rwanda. International Food Research Journal. 2017;24(1):459.
66. Nishimwe K, Wanjuki I, Karangwa C, Darnell R, Harvey J. An initial characterization of aflatoxin B1 contamination of maize sold in the principal retail markets of Kigali, Rwanda. Food Control. 2017;73:574-580.
    CrossRef
67. Umereweneza D, Kamizikunze T, Muhizi T. Assessment of mycotoxins types in some foodstuff consumed in Rwanda. Food Control. 2018;85:432-436.
    CrossRef
68. Udomkun P, Mutegi C, Wossen T, et al. Occurrence of aflatoxin in agricultural produce from local markets in Burundi and Eastern Democratic Republic of Congo. Food Science & Nutrition. 2018;6(8):2227-2238.
    CrossRef
69. Kimanya ME, Shirima CP, Magoha H, et al. Co-exposures of aflatoxins with deoxynivalenol and fumonisins from maize-based complementary foods in Rombo, Northern Tanzania. Food Control. 2014;41:76-81.
    CrossRef
70. Kumar A, Singh B, Raigond P, et al. Phytic acid: Blessing in disguise, a prime compound required for both plant and human nutrition. Food Research International. 2021;142:110193.
    CrossRef
71. Choudhary AK, Kumari P. Management of mycotoxin contamination in preharvest and post-harvest crops: present status and future prospects. Journal of Phytology. 2010;2(7):37-52.
72. Gong YY, Turner PC, Hall AJ, Wild CP. Aflatoxin exposure and impaired child growth in West Africa: an unexplored international public health burden? In: Mycotoxins: Detection Methods, Management, Public Health and Agricultural Trade. CABI Publishing; 2008:53-65.
    CrossRef
73. Kamala A, Kimanya M, Haesaert G, et al. Local post-harvest practices associated with aflatoxin and fumonisin contamination of maize in three agro-ecological zones of Tanzania. null. 2016;33(3):551-559. doi:10.1080/19440049.2016.1138546
    CrossRef
74. Ngure FM, Kassim N, Phillips EL, Turner PC. Infant and Young Child Feeding Practices and Mycotoxins Contamination of Complementary Food Ingredients in Kongwa District, Tanzania. Current Developments in Nutrition. 2023;7:100030.
    CrossRef
75. Nyangi C, Beed F, Mugula J, et al. Assessment of pre-harvest aflatoxin and fumonisin contamination of maize in Babati District, Tanzania. African Journal of Food, Agriculture, Nutrition and Development. 2016;16(3):11039-11053.
    CrossRef
76. Anthony MH, Francis DM, Berka NP, Ayinla GT. Aflatoxin contamination in foods and feeds: A special focus on Africa. In: Ayman AE, ed. Trends Vital Food Control Eng. Books on Demand; 2012.
77. Lahouar A, Marin S, Crespo-Sempere A, Saïd S, Sanchis V. Effects of temperature, water activity and incubation time on fungal growth and aflatoxin B1 production by toxinogenic Aspergillus flavus isolates on sorghum seeds. Revista Argentina de microbiologia. 2016;48(1):78-85.
    CrossRef
78. Ngoma S, Tiisekwa B, Mwaseba D, Kimanya M. Parents’ Practices Associated with Aflatoxin Contamination and Control of Complementary Foods in Central Tanzania. Journal of Food and Nutrition Sciences. 2016;4(6):152-161.
    CrossRef
79. Kaaya AN, Harris C, Eigel W. Peanut aflatoxin levels on farms and in markets of Uganda. Peanut Science. 2006;33(1):68-75.
    CrossRef
80. Hell K, Cardwell KF, Setamou M, Poehling HM. The influence of storage practices on aflatoxin contamination in maize in four agroecological zones of Benin, West Africa. Journal of stored products research. 2000;36(4):365-382.
    CrossRef
81. Aron L, Makangara JJ, Kassim N, Ngoma SJ. Post-harvest Practices Associated with Aflatoxins Contamination of Complementary Flours in Bahi District, Dodoma, Tanzania. International Journal of Sciences: Basic and Applied Research. 2017;36(6):174-186.
82. Villers P. Aflatoxins and safe storage. Frontiers in microbiology. 2014;5:158.
    CrossRef
83. Bernaldez V, Cordoba JJ, Magan N, Peromingo B, Rodriguez A. The influence of ecophysiological factors on growth, aflR gene expression and aflatoxin B1 production by a type strain of Aspergillus flavus. LWT-Food Science and Technology. 2017;83:283-291.
    CrossRef
84. Coppock RW, Christian RG, Jacobsen BJ. Aflatoxins. In: Veterinary Toxicology. Elsevier; 2018:983-994.
    CrossRef
85. Negash D. A review of aflatoxin: occurrence, prevention, and gaps in both food and feed safety. Journal of Applied Microbiological Research. 2018;1(1):35-43.
    CrossRef
86. Li M, Li P, Wu H, et al. An ultra-sensitive monoclonal antibody-based competitive enzyme immunoassay for sterigmatocystin in cereal and oil products. PloS one. 2014;9(9):e106415.
    CrossRef
87. Lamberti I, Tanzarella C, Solinas I, Padula C, Mosiello L. An antibody-based microarray assay for the simultaneous detection of aflatoxin B 1 and fumonisin B 1. Mycotoxin research. 2009;25(4):193-200.
    CrossRef
88. Nelis J, Tsagkaris A, Zhao Y, et al. The end-user sensor tree: An end-user-friendly sensor database. Biosensors and Bioelectronics. 2019;130:245-253.
    CrossRef
89. Turner NW, Subrahmanyam S, Piletsky SA. Analytical methods for determination of mycotoxins: a review. Analytica chimica acta. 2009;632(2):168-180.
    CrossRef
90. Krska R, Welzig E, Berthiller F, Molinelli A, Mizaikoff B. Advances in the analysis of mycotoxins and its quality assurance. Food Additives and Contaminants. 2005;22(4):345-353.
    CrossRef
91. Singh J, Mehta A. Rapid and sensitive detection of mycotoxins by advanced and emerging analytical methods: A review. Food science & nutrition. 2020;8(5):2183-2204.
    CrossRef
92. Yu FY, Vdovenko MM, Wang JJ, Sakharov IY. Comparison of enzyme-linked immunosorbent assays with chemiluminescent and colorimetric detection for the determination of ochratoxin A in food. Journal of agricultural and food chemistry. 2011;59(3):809-813.
    CrossRef
93. Bhat RV, Vasanthi S. Mycotoxin Food Safety Risk in Developing Countries.; 2003.
94. Magoha H, Kimanya M, De Meulenaer B, Roberfroid D, Lachat C, Kolsteren P. Risk of dietary exposure to aflatoxins and fumonisins in infants less than 6 months of age in R ombo, Northern Tanzania. Maternal & child nutrition. 2016;12(3):516-527.
    CrossRef
95. Piacentini KC, Ferranti LS, Pinheiro M, Bertozzi BG, Rocha LO. Mycotoxin contamination in cereal-based baby foods. Current Opinion in Food Science. 2019;30:73-78.
    CrossRef
96. Watson S, Gong YY, Routledge M. Interventions targeting child undernutrition in developing countries may be undermined by dietary exposure to aflatoxin. null. 2017;57(9):1963-1975. doi:10.1080/10408398.2015.1040869
    CrossRef
97. Magoha H, Kimanya M, De Meulenaer B, Roberfroid D, Lachat C, Kolsteren P. Association between aflatoxin M1 exposure through breast milk and growth impairment in infants from Northern Tanzania. World Mycotoxin Journal. 2014;7(3):277-284. doi:10.3920/WMJ2014.1705
    CrossRef
98. Gong YY, Egal S, Hounsa A, et al. Determinants of aflatoxin exposure in young children from Benin and Togo, West Africa: the critical role of weaning. International Journal of Epidemiology. 2003;32(4):556-562. doi:10.1093/ije/dyg109
    CrossRef
99. Gong YY, Watson S, Routledge MN. Aflatoxin exposure and associated human health effects, a review of epidemiological studies. Food safety. 2016;4(1):14-27.
    CrossRef
100. Mollay C, Kimanya M, Kassim N, Stoltzfus R. Main complementary food ingredients contributing to aflatoxin exposure to infants and young children in Kongwa, Tanzania. Food Control. 2022;135:108709.
     CrossRef
101. Obade MI, Andang’o P, Obonyo C, Lusweti F. Exposure of children 4 to 6 months of age to aflatoxin in Kisumu County, Kenya. African Journal of Food, Agriculture, Nutrition and Development. 2015;15(2):9949-9963.
     CrossRef
102. Kiarie G, Dominguez-Salas P, Kang’ethe S, Grace D, Lindahl J. Aflatoxin exposure among young children in urban low-income areas of Nairobi and association with child growth. African Journal of Food, Agriculture, Nutrition and Development. 2016;16(3):10967-10990.
     CrossRef
103. Lauer JM, Duggan CP, Ausman LM, et al. Maternal aflatoxin exposure during pregnancy and adverse birth outcomes in Uganda. Maternal & child nutrition. 2019;15(2):e12701.
     CrossRef
104. Abt. Country and Economic Assessment for Aflatoxin Contamination and Control in Tanzania: Preliminary Findings. Meridian Institute; 2012:1-62. https://archives.au.int/bitstream/handle/123456789/5492/Tanzania%20Country%20Assessment.pdf?sequence=1&isAllowed=y
105. Akumu G, Atukwase A, Tibagonzeka J, et al. On-farm evaluation of effectiveness of improved postharvest handling of maize in reducing grain losses, mold infection and aflatoxin contamination in rural Uganda. African Journal of Food, Agriculture, Nutrition and Development. 2020;20(5):16522-16539.
     CrossRef
106. Massomo SM. Aspergillus flavus and aflatoxin contamination in the maize value chain and what needs to be done in Tanzania. Scientific African. 2020;10:e00606.
     CrossRef
107. Chen C, Mitchell NJ, Gratz J, et al. Exposure to aflatoxin and fumonisin in children at risk for growth impairment in rural Tanzania. Environment international. 2018;115:29-37.
     CrossRef
108. Omara T, Kiprop AK, Wangila P, et al. The scourge of aflatoxins in Kenya: A 60-year review (1960 to 2020). Journal of Food Quality. 2021;2021:1-31.
     CrossRef
109. Aday S, Aday MS. Impact of COVID-19 on the food supply chain. Food Quality and Safety. 2020;4(4):167-180.
     CrossRef
110. Mutiga SK, Were V, Hoffmann V, Harvey JW, Milgroom MG, Nelson RJ. Extent and drivers of mycotoxin contamination: Inferences from a survey of Kenyan maize mills. Phytopathology. 2014;104(11):1221-1231.
     CrossRef
111. Mutiga SK, Mushongi AA, Kangéthe EK. Enhancing food safety through adoption of long-term technical advisory, financial, and storage support services in maize-growing areas of East Africa. Sustainability. 2019;11(10):2827.
     CrossRef
112. Aoun M, Stafstrom W, Priest P, et al. Low-cost grain sorting technologies to reduce mycotoxin contamination in maize and groundnut. Food Control. 2020;118:107363. doi:10.1016/j.foodcont.2020.107363
     CrossRef
113. Mutegi C, Cotty P, Bandyopadhyay R. Prevalence and mitigation of aflatoxins in Kenya (1960-to date). World Mycotoxin Journal. 2018;11(3):341-357.
     CrossRef
114. Gbashi S, Madala NE, De Saeger S, et al. The socio-economic impact of mycotoxin contamination in Africa. Fungi and mycotoxins- their occurrence, impact on health and the economy as well as pre-and postharvest management strategies (ed Njobeh, PB). Published online 2018:1-20.
     CrossRef
115. Mutiga SK, Chepkwony N, Hoekenga OA, Flint‐Garcia SA, Nelson RJ. The role of ear environment in postharvest susceptibility of maize to toxigenic Aspergillus flavus. Plant Breeding. 2019;138(1):38-50.
     CrossRef
116. Nada S, Nikola T, Bozidar U, Ilija D, Andreja R. Prevention and practical strategies to control mycotoxins in the wheat and maize chain. Food Control. 2022;136:108855.
     CrossRef
117. Murage KEW, Madise NJ, Fotso JC, et al. Patterns and determinants of breastfeeding and complementary feeding practices in urban informal settlements, Nairobi Kenya. BMC Public Health. 2011;11(1):1-11.
     CrossRef
118. Kimiywe J, Chege P. Complementary feeding practices and nutritional status of children 6-23 months in Kitui County, Kenya. Journal of Applied Biosciences. 2015;85:7881-7890.
     CrossRef
119. Mutegi C, Ngugi H, Hendriks S, Jones R. Prevalence and factors associated with aflatoxin contamination of peanuts from Western Kenya. International journal of food microbiology. 2009;130(1):27-34.
     CrossRef
120. Kimiywe J, Chege P. Complementary feeding practices and nutritional status of children 6-23 months in Kitui County, Kenya. Journal of Applied Biosciences. 2015;85:7881-7890-7881-7890.
     CrossRef
121. Achieng VO, Kuria FW, Opit S, Muthui S. Complementary feeding practices and nutritional status of infants and young children in Kenya. Nutrients. 2018;10(11):1623.
122. Obonyo N, Kimani MEW, Wachira J. Complementary feeding practices and dietary diversity of infants and young children in Nairobi, Kenya. Food & Nutrition Research. 2019;63:3317458.
123. Omuombo C, Kimani MEW, Wachira, J. Complementary feeding practices and dietary diversity of infants and young children in Nairobi, Kenya. Maternal and Child Nutrition. 2018;14(2):e12519.
124. Muthuri LK, Waititu E, Ndungu P. Complementary feeding practices among mothers in a peri-urban slum in Nairobi, Kenya. Maternal and Child Nutrition. 2018;14(4):e12523.
125. Dusingizimana T, Weber JL, Ramilan T, Iversen PO, Brough L. A qualitative analysis of infant and young child feeding practices in rural Rwanda. Public Health Nutrition. 2021;24(12):3592-3601.
     CrossRef
126. Niyibituronsa M, Onyango A, Gaidashova S, et al. Evaluation of mycotoxin content in soybean (Glycine max l.) grown in Rwanda. African Journal of Food, Agriculture, Nutrition and Development. 2018;18(3):13808-13824.
     CrossRef
127. Thairu L. Achieving optimal infant and young child feeding practices: case studies from Tanzania and Rwanda. Infant and young child feeding: challenges to implementing a global strategy Chichester, United Kingdom: Wiley-Blackwell. Published online 2009:99-118.
128. Uwiringiyimana V, Ocké MC, Amer S, Veldkamp A. Predictors of stunting with particular focus on complementary feeding practices: A cross-sectional study in the northern province of Rwanda. Nutrition. 2019;60:11-18.
     CrossRef
129. Ahishakiye J, Bouwman L, Brouwer ID, Matsiko E, Armar-Klemesu M, Koelen M. Challenges and responses to infant and young child feeding in rural Rwanda: a qualitative study. Journal of Health, Population and Nutrition. 2019;38(1):1-10.
     CrossRef
130. Grosshagauer S, Milani P, Kraemer K, et al. Inadequacy of nutrients and contaminants found in porridge‐type complementary foods in Rwanda. Maternal & Child Nutrition. 2020;16(1):e12856.
     CrossRef
131. Mohammed SGS. Infants feeding and weaning practices among mothers in northern Kordofan state, Sudan. European Scientific Journal. 2014;10(24):165-181.
132. Tongun JB, Sebit MB, Ndeezi G, Mukunya D, Tylleskar T, Tumwine JK. Prevalence and determinants of pre-lacteal feeding in South Sudan: a community-based survey. Global Health Action. 2018;11(1):1523304. doi:10.1080/16549716.2018.1523304
     CrossRef
133. Mbela DEN. Modified Diets to Improve Iron, Vitamin A and Protein Intake among Children in Banana-Based Farming Systems of Kagera Region, Tanzania. Sokoine University of Agriculture; 2018.
134. Lyimo M, Muzanila Y. Assessment of complementary foods and child feeding practices in Dodoma Region, Tanzania. Published online 2014.
135. Mgongo M, Hussein TH, Stray-Pedersen B, Vangen S, Msuya SE, Wandel M. “We give water or porridge, but we don’t really know what the child wants:” A qualitative study on women’s perceptions and practises regarding exclusive breastfeeding in Kilimanjaro region, Tanzania. BMC pregnancy and childbirth. 2018;18(1):1-9.
     CrossRef
136. Mgongo M, Hussein TH, Stray-Pedersen B, Vangen S, Msuya SE, Wandel M. Facilitators and barriers to breastfeeding and exclusive breastfeeding in Kilimanjaro region, Tanzania: a qualitative study. International Journal of Pediatrics. 2019;2019.
     CrossRef
137. Tesha AP. Improving Nutrient Content of the Frequently Used Complementary Foods for Children Aged 6-23 Months in Rombo District, Kilimanjaro. Sokoine University of Agriculture; 2017.
138. Mollay C, Kimanya M, Kassim N, Stoltzfus R. Main complementary food ingredients contributing to aflatoxin exposure to infants and young children in Kongwa, Tanzania. Food Control. 2022;135:108709.
     CrossRef
139. Masuke R, Msuya SE, Mahande JM, et al. Effect of inappropriate complementary feeding practices on the nutritional status of children aged 6-24 months in urban Moshi, Northern Tanzania: Cohort study. PloS one. 2021;16(5):e0250562.
     CrossRef
140. Muhihi AJ, Lyimo J. Complementary feeding practices and associated factors among mothers in rural Tanzania. Maternal and Child Nutrition. 2017;13(2):e12307.
141. Mushi AK, Mamiro PS, Mugyabuso M. Complementary feeding practices and dietary diversity of infants and young children in Tanzania. Maternal and Child Nutrition. 2017;13(2):e12300.
142. Asiki G, Seeley J, Srey C, et al. A pilot study to evaluate aflatoxin exposure in a rural Ugandan population. Tropical Medicine & International Health. 2014;19(5):592-599.
     CrossRef
143. Atukwase A, Kaaya AN, Muyanja C. Dynamics of Fusarium and fumonisins in maize during storage–a case of the traditional storage structures commonly used in Uganda. Food control. 2012;26(1):200-205.
     CrossRef
144. Ssemukasa EL, Kearney J. Complementary feeding practices in Wakiso district of Uganda. African Journal of Food, Agriculture, Nutrition and Development. 2014;14(4):9085-9103.
     CrossRef
145. Nassanga P, Okello‐Uma I, Ongeng D. The status of nutritional knowledge, attitude and practices associated with complementary feeding in a post‐conflict development phase setting: The case of Acholi sub‐region of Uganda. Food science & nutrition. 2018;6(8):2374-2385.
     CrossRef
146. Namakula J, Ndyanabangi S. Complementary feeding practices and dietary diversity of infants and young children in rural Uganda. Maternal and Child Nutrition. 2017;13(3):e12297.
147. Anitha S, Muzanila Y, Tsusaka TW, et al. Reducing child undernutrition through dietary diversification, reduced aflatoxin exposure, and improved hygiene practices: The immediate impacts in central Tanzania. Ecology of food and nutrition. 2020;59(3):243-262.
     CrossRef
148. Okoth SA, Ohingo M. Dietary aflatoxin exposure and impaired growth in young children from Kisumu District, Kenya: Cross-sectional study. African journal of health sciences. 2004;11(1):43-54.
     CrossRef
149. Mutegi C, Ngugi H, Hendriks S, Jones R. Prevalence and factors associated with aflatoxin contamination of peanuts from Western Kenya. International journal of food microbiology. 2009;130(1):27-34.
     CrossRef
150. Ankwasa E, Francis I, Ahmad T. Update on mycotoxin contamination of maize and peanuts in East African Community Countries. J Food Sci Nutr Ther. 2021;7:1-10.
151. Mollay C, Kassim N, Stoltzfus R, Kimanya M. Complementary feeding in Kongwa, Tanzania: Findings to inform a mycotoxin mitigation trial. Maternal & Child Nutrition. Published online 2021:e13188.
     CrossRef
152. Kamala A, Shirima C, Jani B, et al. Outbreak of an acute aflatoxicosis in Tanzania during 2016. World Mycotoxin Journal. 2018;11(3):311-320.
     CrossRef
153. Menza NC, Margaret MW, Lucy KM. Incidence, types and levels of aflatoxin in different peanut varieties produced in Busia and Kisii Central Districts, Kenya. Open Journal of Medical Microbiology. 2015;5(04):209.
     CrossRef
154. Matsiko F, Kanyange C, Ingabire G, Dusingizimana T, Vasanthakaalam H, Kimonyo A. Detection and quantification of aflatoxin in cassava and maize flour sold in Kigali open markets, Rwanda. International Food Research Journal. 2017;24(1):459.
155. Lee S, RDA S, Lee S, et al. Survey for contamination of aflatoxin in Uganda maize. The Journal of the Korean Society of International Agriculture. Published online 2013.
     CrossRef
156. Makori N, Matemu A, Kimanya M, Kassim N. Inadequate management of complementary foods contributes to the risk of aflatoxin exposure and low nutrition status among children. World Mycotoxin Journal. 2019;12(1):67-76.
     CrossRef
157. Kiarie G, Dominguez-Salas P, Kang’ethe S, Grace D, Lindahl J. Aflatoxin exposure among young children in urban low-income areas of Nairobi and association with child growth. African Journal of Food, Agriculture, Nutrition and Development. 2016;16(3):10967-10990.
     CrossRef
158. Kang’ethe EK, Gatwiri M, Sirma AJ, et al. Exposure of the Kenyan population to aflatoxins in foods with special reference to Nandi and Makueni counties. Food Quality and Safety. 2017;1(2):131-137. doi:10.1093/fqsafe/fyx011
     CrossRef
159. Nabwire WR, Ombaka J, Dick CP, et al. Aflatoxin in household maize for human consumption in Kenya, East Africa. Food Additives & Contaminants: Part B. 2020;13(1):45-51.
     CrossRef
160. Niyibituronsa M, Mukantwali C, Nzamwita M, et al. Assessment of aflatoxin and fumonisin contamination levels in maize and mycotoxins awareness and risk factors in Rwanda. African Journal of Food, Agriculture, Nutrition and Development. 2020;20(5):16420-16446.
     CrossRef
161. Mollay C. Complementary Feeding Practices and the Risk of Exposure to Aflatoxins among Infants and Young Children in Kongwa, Tanzania. NM-AIST; 2022.
     CrossRef
162. Shirima CP, Kimanya ME, Routledge MN, et al. A prospective study of growth and biomarkers of exposure to aflatoxin and fumonisin during early childhood in Tanzania. Environmental health perspectives. 2015;123(2):173-178.
     CrossRef
163. Kimanya ME, Meulenaer BD, Baert K, et al. Exposure of infants to fumonisins in maize‐based complementary foods in rural Tanzania. Molecular nutrition & food research. 2009;53(5):667-674.
     CrossRef
164. Hoffmann V, Moser C, Saak A. Food safety in low and middle-income countries: The evidence through an economic lens. World Development. 2019;123:104611.
     CrossRef
165. Rwanda Standards Board. Fortified Processed Foods (FPF)—Specification. Rwanda Standards Board; 2016.
166. Kumar P, Kamle M, Mahato DK. Mycotoxins in Food and Feed: Detection and Management Strategies. (Kumar P, Kamle M, Kumar M, eds.). CRC Press; 2023.
     CrossRef
167. Azziz-Baumgartner E, Lindblade K, Gieseker K, et al. Case–control study of an acute aflatoxicosis outbreak, Kenya, 2004. Environmental health perspectives. 2005;113(12):1779-1783.
     CrossRef
168. Wokorach G, Landschoot S, Anena J, Audenaert K, Echodu R, Haesaert G. Mycotoxin profile of staple grains in northern Uganda: Understanding the level of human exposure and potential risks. Food Control. 2021;122:107813.
     CrossRef

Appendixes

Table 1: Common ingredients of CFs in the East African region

Table 2: Contamination of complementary foods with AFs and FBs in the East African region

TLC = Thin Layer Chromatography, ELISA = Enzyme-linked Immunosorbent Assays, HPLC = High-Performance Liquid Chromatography

Table 3: Exposure of infants and young children to aflatoxins and fumonisins in the East African region