Introduction 

Hypertension remains the most common risk factor in cardiovascular disease¹. Hypertension is a clinical condition characterized by an elevation of systolic blood pressure above 140 mmHg and diastolic blood pressure above 90 mmHg².

According to the World Health Organization (WHO), around 1.28 billion adults aged 30-79 years worldwide suffer from hypertension, most of them live in low- and middle-income countries. One of the global targets for non-communicable diseases is to reduce the prevalence of hypertension by 33% between  2010  and  2030 ³.

According to the 2018 Basic Health Research (Riskesdas) in Indonesia, the incidence of hypertension among the population aged >18 years was 658.201 diagnosed with hypertension, with a measured prevalence of 34.1%, this represents an increase from 25.8% in 2013. The province with the highest prevalence of hypertension is South Kalimantan at 44.1%, while the province with the lowest prevalence is Papua at 22.2%⁴. According to the health profile data of Bengkulu City, the estimated number of hypertension patients in Bengkulu City in 20l22 was 36.404 people, with 14.812 people (40,7%), receiving standard care. The highest number of cases was found in the working area of the Telaga Dewa Community Health Center, with 3.852 people, and a coverage of those receiving health services at 20.1%⁵.

Hypertension, if not treated and tackled, will cause various complications in the long run. Hypertension is a risk factor for diseases such as heart disease, kidney failure, diabetes, stroke⁶.

Hypertension treatment can be done in 2 ways, namely pharmacological and non-pharmacological methods. Pharmacologically treatment is by using anti-hypertensive drugs, such as diuretics, beta blockers, ACE inhibitors, ca blocker, Meanwhile, non-pharmacological treatment is reducing stress, weight loss, increasing physical activity and exercise, limiting the consumption of alcohol, sodium and cigarettes, modifying food diet, stopping smoking habits⁷. In addition, the use of natural ingredients (herbs) such as traditional medicinal plants or plants that have been clinically and preclinically tested can lower blood pressure⁸. Natural (herbal) ingredients with the development of functional foods are favored by the public due to their practicality and easy availability. Functional foods that have been used for hypertension include roselle flowers, honey, and dates.

Roselle flowers (Hibiscus sabdariffa L) are one of the traditional alternative remedies for lowering blood pressure. The active compounds in roselle flowers that can reduce blood pressure are anthocyanins, phenolics, theophylline, leucine, valine, glycine, and ascorbic acid. These active compounds can help improve blood circulation by reducing the degree of blood viscosity, thereby reducing the workload on the heart and lowering blood pressure ⁹. According to Wijaya et al, the infusion of 10 g of roselle petals in 200 ml of hot water can reduce blood pressure, namely the normal blood pressure category totaling 10 respondents (30.3%) and the 1st degree hypertension category totaling 23 respondents (69.7%)⁹.

Honey is one of the functional foods that has been traditionally believed to lower blood pressure. The study by Kharisma found a decrease in blood pressure in the elderly with the administration of 20 ml of honey in the morning and evening for 7 days, with values decreasing from 158/95 mmHg to 138/90 mmHg¹⁰.

Dates are the main source of energy for people living in Saudi Arabia. The use of dates (Phoenix dactylifera L) as a medicinal plant, and a spice in the treatment of various diseases. Ajwa dates are also high in potassium and low in sodium and therefore can be included in the DASH (Dietary approaches to stop hypertension)¹¹. The results of other research showed that there was a difference in the reduction of systolic and diastolic blood pressure between the treatment group and the control group, proving that the administration of ajwa dates 100 g/day for 6 weeks had an effect on blood pressure in the elderly¹².

Many studies have used roselle flowers, honey and dates separately as alternatives in lowering blood pressure, so far no researchers have examined whether the combination of these three ingredients can have a better effect on lowering blood pressure. Based on this description, the researcher is interested in researching the effect of giving boiled water of roselle flowers combined with honey and dates on blood pressure in hypertensive patients in the working area of the Telaga Dewa Health Center, Bengkulu City in 2024.

Materials and Methods

Materials preparation

The ingredients used are roselle flowers, honey and ajwa dates. The preparation of ingredients is carried out by the first way of washing the roselle flowers, the second weighing each ingredient according to the dose given, the third is boiling the roselle for 15 minutes, the fourth is the addition of honey and date juice with each dose. The dose used is roselle flowers with each treatment dose of 10 g, 30 g and 50 g roselle flowers, 20 ml of honey, 50 g ajwa dates brewed 20l0 ml of hot water.

Preparation of Roselle Flower Decoction

Firstly, prepare all the ingredients and do the weighing according to the treatment group. Secondly, wash the roselle flower ingredients. Thirdly, boil or cook roselle flowers with 300 ml of water to boil. Fourthly, cool the roselle flower  stew to cool and then put it in a 200 ml bottle and add 50 ml date juice and 20 ml honey. Finally, the roselle flower stew is ready to serve.

Design and Sampling Techniques

This type of research is an experimental research with  a group randomized design research design (RAK) see in figure 1, namely: with a combination of 4 treatments, namely treatment 1 was given a dose of roselle 10l g, treatment 2 was given a dose of roselle 30 g, treatment 3 was given a dose of roselle 50l g and the control treatment was only given leaflets.  The sample appearance technique is purposive sampling, the number of samples in this study is 28 people with hypertension, The determination of sample size is carried out using the Federer formula (1991)¹³ :

(k-1) (r-1) ≥ 15

Information:

k= number of samples searched

r = number of treatments

In this study, it is known that there were 4 treatments (r) = 4, namely 1 control group and 3 treatment groups, so that the n value was obtained as follows: (k-1)(r-1)  = 15

(k-1)(4-1)  = 15

(k-1)(3)     = 15

3k-3          = 15

3k             = 18

k               = 6

Based on calculations using the Federer formula, the number of samples according to the data above is 6 respondents for each treatment group. As for the calculation of the drop out, it is 1 respondent. So, he sample size in this study is 7 people in each group so that a total of 28 respondents are obtained.

Figure 1 Schematic of The Experimental Design.  Click here to view Figure

Location and Time of Research

The research was carried out in the working areal of the Telaga Dewa health center, Bengkulu city, The research time was carried out for 1 week from April 29, 2024 – May 9, 2024.

Data Collection and Data Analysis Techniques

The types of data used are primary data and secondary data. Primary data collection with the target of pre-elderly, age range of 45-59 years. The research instrument used food recall for 3 days.  Analyze nutritional value using the nutrisurvey application.  The tool used to measure blood pressure is the Omicron brand digital blood pressure monitor.  The data taken included name, age, gender, and address.  Secondary data in the form of data on the highest prevalence of hypertension obtained from the City Health Office and an integrated recording and reporting system book for hypertension patients alt the Telaga Dewa Health Center in the Telaga Dewa Health Center areal, Bengkulu City. Data analysis was determined by univariate analysis which included numerical data using mean or average, median, maximum  and  minimum values as well as standard deviations, while bivariate analysis used statistical tests using SPPS version 22 made in America (2013) using the anova test, if related then continued with the Duncan test to see which treatment group was most significant in reducing blood pressure.

Results

The characteristics of hypertensive patient respondents in this study are age, sex, occupation and education. These results can be seen in table 1. 

Table 1: Characteristics Responding

Note:

IRT : Housewife

SD : Elementary School

SMP: Junior High School

SMA: Senior High School

Based on table 1, The characteristics of the respondents showed that most of the age of hypertension sufferers was 50-64 years old (57.1%), most of the gender of the respondents (75%) were female, the education of the respondents was mostly high school (64.3%) and the occupation of most housewives (64%).

Table 2: Blood Pressure Picture Before and After Treatment

Source: Descriptive Statistics

This study states that the average results of blood pressure in each treatment group, the control group was given a leaflet the results before the intervention were 157/91 mmHg and after the intervention were 153/91 mmHg. In treatment group 1 given a dose of roselle flowers 10 gr the results before the intervention were 153/97 mmHg and after the intervention were 142/85 mmHg. While the treatment group 2 was given a dose of 30 gr roselle flowers, the results before the intervention were 149/98 mmHg and after the intervention were 137/84 mmHg. Finally, treatment group 3 was given a dose of 50 grams of roselle flowers, the results before the intervention were 156/99 mmHg and after the intervention were 120/81 mmHg.

Table 3: The Effect of Al Decoction of Roselle Flowers Combined With Honey alnd Daltes on Hypertension Sufferers

Source : Anova statistics

Based on the results of the ANOVA one-way test, it was shown that the combination of roselle flower boiled water, honey and dates on blood pressure for 7 days had a real effect on the reduction of systolic and diastolic blood pressure in hypertensive patients alt the Telaga Dewa Health Center in this study. This can be seen in table 3 (p<0.05). Based on the results of the anova test all significance values obtained results, namely, systolic blood pressure before is p<0.029, systolic blood pressure after is p<0.000, diastolic blood pressure before is p<0.049, diastolic blood pressure after is p<0.004

Table 4: The Best Treatment Among the 4 Treatments

Source: Duncan Statistics

To find out the difference between each treatment, it can be known with al real difference test where in this study the Duncan test technique was chosen. Based on the real difference test or Duncan  test, it was shown that the four treatments, namely control (P0), treatment 1 (P1), treatment 2 (P2), treatment 3 (P3) had al real effect on the reduction of systolic and diastolic blood pressure in hypertensive patients in the Telaga Dewa health center areal. It can be seen in the post-systolic and diastolic tables that treatment 3 is in first order which shows that the third treatment is the best treatment with systolic blood pressure of 120 mmHg and diastolic 81 mmHg. In treatment 2 was in second place and treatment 1 was in third place with systolic blood pressure of 137 mmHg and 142 mmHg while diastolic 84 mmHg and 85 mmHg showed that treatment 1 and 2 were not better than treatment 3, but compared to control treatment 1 and 3 were better than treatment of the control group.

Discussion

The research results showed that the majority of respondents were 50l-64 years old, with a total of 16 people (57%). In line with research Nuraeni the results of the study show that those with an older age (≥ 45 years) are more alt risk of suffering from hypertension when compared to those who are young (<45 years old)¹⁴. (see table 1)

The gender of respondents is mostly female (75%). In line with research Diba et al of the 68 samples, it is known that the gender group that experienced hypertension was mostly women, namely 37 respondents or 54.5%¹⁵. The results of this research are supported by several studies such as those found by Irawan et al women will experience an increased risk of high blood pressure (hypertension) after menopause, which is over the age of 45 years. Women who halve not yet menopausal are protected by the hormone estrogen which plays al role in increasing HDL (High Density Lipoprotein) levels. Therefore, when women halve menopause, they will be equally alt risk of developing hypertension¹⁶. ( see table 1)

The education of the respondents was mostly high school education 64% (18 people). In line with the research of Sutrisno et al the level of education has al strong influence on hypertension control behalvior¹⁷. (see table 1)

Most of the respondents’ jobs were housewives as many as 16 people (64%)  In line with research conducted by Susanti et al the work of the majority of respondents (38.6%), many housewives who suffer from hypertension complain of lack of exercise, because they are busy talking care of their homes and children so that they do not halve free time to do sports and the many mental burdens that cause high blood pressure to be difficult to control¹⁸. (see table 1)

This study was divided into 4 treatment groups, namely group P0 (control group given education), group P1 (roselle flower dose group 10 g), group P2 (roselle flower dose group 30 g), and group P3 (roselle flower dose group 50 g). In each group, each treatment consisted of 7 respondents. (see in figure 1)

The results of the anova test showed the difference in the average blood pressure values of the respondents in each treatment group after being given the intervention between the control treatment, treatment 1, treatment 2, and treatment 3. From all treatment groups, systolic blood pressure p value 0.00l0 (<0.05) and diastolic blood pressure p value 0.004 (<0.05) showed that there was al significant difference in each treatment group after the intervention (see table 2).  However, the blood pressure sequence has not reached the normal limit because there are many factors that affect it, namely during the study there were still many respondents who consumed salty foods whose sodium    levels were quite high which could cause unstable blood pressure and were not accompanied by healthy food as evidenced by the results of the recall for 3 non-consecutive recalls, the average recall was the first day of 1890 mg,  The second day was 1737 mg and the third day was 1875 mg higher compared to the daily sodium AKG which was 150l0l mg. In addition, the length of the study and lack of physical activity such as exercise can also affect blood pressure. In line with research conducted by Jenti Sitorus that there is al relationship between salt intake and the incidence of hypertension with al p-value of 0.021 (p< 0.05)¹⁹. Poor salt intake has al 3 times greater risk of developing hypertension than patients with good salt intake. In line with the research of Suzana et al showed that sodium intake is one of the important factors affecting hypertension control, with subjects consuming sodium more than 2400 mg/day alt al triple risk of developing uncontrolled hypertension²⁰. In line with research conducted by  Malt Nasir et al found that those who suffer from hypertension halve al much lower potassium intake (2.3 g/day) than those who do not suffer from hypertension (2.4 g/day), even though low potassium intake can also cause the risk of hypertension. Potassium, which plays an important role in cell metabolism as well as electrolyte and fluid balance, affects blood pressure in the opposite way to sodium due to the action of the sodium-potassium   pump²¹.

The results of the test continued Duncan to find out in detail which data groups differed significantly. Based on the study, it was shown that the best dose was 50l g of roselle, 20l ml of honey and 50   g of dates in the treatment group 3. This research is in line with the research  of Wijaya et al in Rohaendi, H. (20l0l8) that roselle flowers can lower blood pressure, roselle flowers (Hibiscus sabdariffa) in lowering blood pressure because they contain anthocyanins that halve the ability to maintain the elasticity of blood vessel walls, the more elastic the blood vessel walls, the smaller the systemic resistalnce⁹. In line with the research  of Melani and Eka Sudiarti  the  administration of roselle flowers was obtained as al result of the effect of giving roselle flower decoction, blood pressure in the elderly with al ratio of TD: 183/98 mmHg decreased until the third day obtained TD: 168/94 mmHg²². In line with research conducted by Ahad et al Hibiscus sadariffa L given to hypertensive rats as much as 250l mg for 2 weeks can provide the effect of hypotension losalrtaln²³. The anti-hypertensive activity of roselle may be related to various synergistic mechanism\s such as diuretic effects and blocking of the renin-angiotensin-aldosterone systeml²⁴.

Another study stated that roselle sabdariffa flowers contain anthocyanins which was phenolic compounds from flavonoids and one of the water-soluble pigments in plants.

These compounds contribute to Hibiscus sabdariffa L ability  to work als antihypertensive, antihyperlipidemia, antiobesogenic, antidiabetic, and all produce anti-Alzheimer’s natural products ²⁵.  In addition, roselle also contains tocopherols known as biological antioxidants that can prevent or slow down the oxidation of body lipids, which include polysaturated fatty acids and cellular lipid components ²⁶. The main anthocyanins are hibiscin and gossypicyanin which caln lower blood pressure ²⁷. Based on research by Efosa et al dried flower petal extract alt al temperature of 30^◦C shows higher antioxidant and antihypertensive potentiall²⁸. Based on research conducted by Nurfaradilla et al the administration of roselle flower extract can produce antihypertensive activity, for 2 weeks of administration of rosella flower extract before the administration of katopri can significantly affect the pharmacokinetics of kaltopril²⁹. In line with the research of Al-Anbaki et al that hibiscus sabdariffa L (roselle flower) with adjusted doses (10l, 15, and 20 g daily according to clinical response) for 4 weeks can lower high blood pressure in some patients with or without antihypertensive drugs³⁰. In addition, al study conducted  by Elkalfrawy et al that the combination of rosella flower and olea europaea leaf extract  showed comparable antihypertensive and safety in patients newly diagnosed with grade 1 hypertension³¹. Based on research by Salem et al that hot rose flower extract produces antihypertensive effects through al decrease in plasma ACE, angiotensin II, and aldosterone levels³².

In addition, honey is beneficial in preventing hypertension through its antioxidant and anti-inflammatory effects ³³.  One of the anti-oxidants of honey is flavonoids, flavonoids reduce Systemic Vascular Resistance (SVR) and affect the work of Angiotensin Converting Enzyme (ACE) which is able to inhibit the change of angiotensin I to angiotensin II. The vasodilation effect and  ACE inhibitors lower blood pressure³⁴. Flavonoids halve been linked to diuretic effects on honey ³⁵.  Flavonoids found in honey, such as quercetin and kaempferol, showed results in cardiovascular treatment, treatment with 10 mg/kg quercetin for 13 weeks lowered blood pressure and heart rate in hypertensive rats ³⁶.  In line with research conducted by Kharisma Niza et al the results of the study, there was al decrease in blood pressure in the elderly by giving honey in the morning and evening alt al dose of 20 ml for 7 days with values before 158/95 mmHg and values after 138/90 mmHg¹⁰. Another study conducted  by Musyayyadah et al showed al significant effect of al honey solution  intervention with honey doses of 35 and 70 g on systolic and diastolic blood pressure in the elderly³⁷. Based on the research of Ramli et al that there is al high concentration of nitrous oxide (NO) vasodilating agent in honey that contributes to the therapeutic effect on hypertension³³.

One of the fruits that can lower high blood pressure is dates, ajwa dates contain carbohydrates, dietary fiber, fats and various types of amino acids and vitamins, halve anti-free radical, antioxidant, antimutagenic, antimicrobial, anticancer and immunostimuant activities. Antioxidant activity is caused by al variety of phenolic compounds, alkaloids, sterols and flavonoids found in ajwa dates³⁸. The content of flavonoids includes quercetin, orientin, and flavanon. The flavonoid content in 100 grams of dates analyzed varied greatly and ranged from 68.88 to 208.53 mg RE ¹².  Dates are al rich source of phenolics and flavonoids so they halve al potential role in protecting against cell damage caused by oxidative stress produced by free radicals produced in the body³⁹.  Dates contain gallic acid which is known as an antioxidant compound  that functions to reduce chronic stress and pathogens such as cancer, degenerative diseases and metabolic diseases.  In addition, according to research conducted by Alam et al dates also contain melanin, date melanin shows an ACE inhibitory effect⁴⁰. In line with the research of Husaidah et al consuming dates for 30 days with al dose of 7 grains/day (100 gr/day) with the results of the study there was al significant difference between  before and after the administration of  ajwa dates in al positive direction⁴¹. Giving ajwa dates can affect changes in blood pressure.  In line with the research of  Syafriati alnd Ana that there is an effect of giving date juice on reducing blood pressure in elderly patients alt the Pengarayan Health Center in 2023, this is because dates have potassium content, al food that is high in potassium can stabilize blood pressure⁴².  In line with the research of Pratiwi et al  that the respondents potassium levels  increased after consuming date infused water for 7 dalys⁴³.

Micronutrients that play an important role in the development of hypertension are partly due to excessive intake of sodium (Na) in al certain amount of time and also due to an imbalance in potassium (K) intalke⁴⁴. Excessive sodium consumption causes the sodium composition in extracellular fluids to increase. The increase in the volume of extracellular fluid causes an increase in blood volume, which has an impact on the onset of hypertension⁴⁵. Potassium intake that is in accordance with the recommended  minimum  potassium requirement in al day can lower blood pressure in women suffering from hypertension with mild to moderate categories ⁴⁴. Low potassium intake can result in an increase in blood pressure, and vice versa with al high intake of potassium can cause al decrease in blood pressure due to al decrease in vascular resistance ⁴⁶.  Damage to blood vessels can be prevented by consuming fiber.  Dietary fiber can help increase cholesterol excretion through feces by increasing the transit time of foodstuffs through the small intestine. In addition, the consumption of vegetable and fruit fiber will accelerate satiety. This situation is advantageous because it can reduce energy intake and obesity, and will ultimately reduce the risk of hypertension ⁴⁷.

Conclusion

This study concluded that the average blood pressure results in each treatment group, the control group given leaflets before the intervention was 157/91 mmHg and after the intervention was 153/91 mmHg. In treatment group 1 which was given a dose of 10 gr of roselle flowers before the intervention was 153/97 mmHg and after the intervention was 142/85 mmHg. While treatment group 2 which was given a dose of 30 gr of roselle flowers before the intervention was 149/98 mmHg and after the intervention was 137/84 mmHg. Finally, the best treatment treatment group 3 was given a dose of 50 gr of roselle flowers, before the intervention was 156/99 mmHg and after the intervention was 120/81 mmHg. With the results of the ANOVA test, namely that there was a significant difference between the four treatments, the results obtained were systolic blood pressure p value 0.000 (<0.05) and diastolic blood pressure p value 0.004 (<0.05), so it can be concluded that each treatment group after the intervention had a significant difference.

Acknowledgement

Thanks for the solid cooperation to all researchers involved in this research Current Research in Nutrition and Food Science, An International, Open Access, Peer Reviewed Research Journal of Nutrition and Food

Funding Sources

This research was funded by Poltekkes Kemenkes Kesehatan Bengkulu: grand number HK.02.03/F.XXXI/606/2024,dated January, 25 th 2024.

Conflict of Interest

All of authors declare there is no conflict of interest.

Authors’ Contribution

Retno Aprillia: als the main researcher, conceptualizing and designing research, preparing manuscript drafts and reviewing manuscripts.

Tonny Cortis Maigoda: as Corresponding author analyzing data, translating from Indonesian to English.

Desri Suryani: . correspondence to journal editors.

Anang Wahyudi: making manuscript layout.

Ahmad Rizal: correcting word spelling, editing the final stage of the manuscript.

Data Availability Statement

Data within the article will be provided upon al justified request. Raw data was available in our research report and originated

Ethics Statement

This ethical statement is valid for the period from March 1, 2024 to March 1, 20l25, ethical number: No.KEKP.BKL/048/03/2024. Issued by the Bengkulu Ministry of Health Polytechnic.

References

1. Ghorani H Al, Götzinger F, Böhm M, Mahfoud F. Arterial hypertension e Clinical trials . Nutr Metab Cardiovasc Dis. 2021;32(1):21-23. doi: https://doi.org/10.1016/j. numecd.2021.09.007
2. PERHI. Hypertension Management Consensus 2019. (Lukito AA, Harmeiwaty E, Hustrini NM, eds.).; 2019. http://faber.inash.or.id/upload/pdf/article_ Update_konsensus_201939.pdf
3. WHO. World Health Organization (WHO) : Hypertension. https://www.who.int/news-room/fact-sheets/detail/hypertension. Published 2023. accessed on May 5, 2024
4. Riskesdas. Riskesdas Report Ministry of Health of the Republic of Indonesia 2018 Riskesdas National Report. 2018 ; 53 (9) : 154-165. http://www.yankes.kemkes.go.id/assets/downloads/PMK No. 57 Year 2013 on PTRM.pdf.Accesed on May 7,2024
5. Bengkulu Provincial Health Office. Health Profile of Bengkulu Province Year 2022; 2022.dinkes.bengkuluprov.go.id.Accesed on May 27,2024.
6. Ministry of Health. Hypertension is the most common disease in the community. Indonesian Ministry of Health. Published 2019. https://www.kemkes.go.id/id/rilis-kesehatan/hipertensi-penyakit-paling-banyak-diidap-masyarakat. Accesed on May 20,2024.
7. Nurhusna, Oktarina Y, Sulistiawan A. The Effect of Laughter Therapy on Decreasing Blood Pressure of Hypertension Patients at the Olak Kemang Health Center, Jambi City. Applied Science Jambi University. 2018 ; 1 (1) : 430-439. doi:https://doi.org/10.22437/jiituj.v2i1.5654
8. Khasanah UN, Imandiri A, Adianti M. Hypertension therapy with acupuncture and celery and carrot herbs. J Vocat Heal Stud. 2019;2(2):67. doi:https://doi.org/10.20473/jvhs.V2I2.2018.67-73
9. Wijaya IPA, Atmaja IKW, Sri1 KI. The Effect of Rosella Flower Decoction (Hisbiscus Sabdariffa) on Lowering Blood Pressure in Patients with Hypertension. Nursing Media of Makassar Health Polytechnic. 2020 ; 11 (1) : 35. doi:https://doi.org/10.32382/jmk.v11i1.1527
10. Kharisma Niza, Sudiarto, Puspasari FD. Giving Pure Honey in Lowering Blood Pressure in Elderly Hypertension. Multidisciplinary Science. 2023;1(7):610-614. doi:https://doi.org/10.5281/zenodo.8299955 Giving
11. Zulfahmidah Z, Sri Wahyuni. M R, F.Bustan A. Effectiveness of Ajwa dates in various diseases. Indones journal of Health. 2021 ; 2 (01) : 18-30. doi:https://doi.org/10.33368/inajoh.v2i1.22
12. Prayoga EA, Nugraheni A, Probosari E, Syauqy A. The Effect of Giving Ajwa Dates (Phoenix Dactylifera) on Blood Pressure in the Elderly. Journal of Nutrition College. 2022;11(1):87-97. doi:https://doi.org/10.14710/jnc.v11i1.32573
13. Federer, W.T. (1991) Statistics and Society : Data Collection and Interpretation. New York: Mercel Dekker.
14. Nuraeni E. The Relationship of Age and Gender at Risk with the Incidence of Hypertension at Clinic X Tangerang City. JKTF Journal: University of Muhammadiyah Tangerang. 2019;4(1):1. doi:https://doi.org/10.31000/jkft.v4i1.1996
15. Diba F, Haningsih S, Pangestuti D, Nasution HT. Analysis of High Sodium Foods on the Incidence of Hypertension in Pre-Elderly and Elderly at Puskesmas Medan Area Selatan. Njm. 2023;9(1):1-5. doi:https://doi.org/10.36655/njm.v9i1.1156
16. Irawan D, Siwi AS, Susanto A. Factors Affecting the Incidence of Hypertension. Journal Amanah Health. 2020;2(1):1-11. doi:https://doi.org/10.55866/jak.v2i1.33
17. Sutrisno, Widayati CN, Radate. The Relationship between Education Level and Attitude towards Hypertension Control Behavior in the Elderly. Shine Light of the Ners World. 2018;3(2):16-27. doi:http://dx.doi.org/10.35720/tscners.v3i2.121
18. Susanti S, Bujawati E, Sadarang RA insani, Ihwana D. The Relationship between Self Efficacy and Self-Management of Hypertension Patients at Puskesmas Kassi-Kassi Makassar City in 2022. Journal Kesmas Jambi. 2022;6(2):48-58. doi: https://doi.org/10.22437/jkmj.v6i2.20540
19. Jenti Sitorus. Effect of diet and physical activity on the incidence of hypertension in outpatients at rsu hkbp balige. Journal of Midwifery Science IMELDA. 2019;5(1):34-43. Scientific Journal of Midwifery Imelda (uimedan.ac.id)
20. Suzana S, Azlinda A, Hin SL, et al. Influence of food intake and eating habits on hypertension control among outpatients at a government health clinic in the Klang Valley, Malaysia. Malays J Nutr. 2011;17(2):163-173.
21. Mat Nasir N, Md Isa Z, Ismail NH, et al. A cross-sectional analysis of the PURE study on minerals intake among Malaysian adult population with hypertension. Scientific Reports. 2024;14(1):1-9. doi:https://doi.org/10.1038/s41598-024-59206-0
22. Melani L, Eka Sudiarti P. Nursing Care for Mr. H’s Family. H With the Giving of Rosella Flower Decoction Water Towards Lowering Blood Pressure in Hypertension Patients in Simpang Kubu Village, Upt Work Area of the Air Tiris Health Center in 2023. Healthy: Integrated Health Journal. 2024;3 (1)(2774-5848):150-160. doi:https://doi.org/10.31004/sjkt.v3i1.25166
23. Ahad A, Raish M, Bin Jardan YA, Alam MA, Al-Mohizea AM, Al-Jenoobi FI. Effect of Hibiscus sabdariffa and Zingiber officinale on the antihypertensive activity and pharmacokinetic of losartan in hypertensive rats. Xenobiotica. 2020;50(7):847-857. doi:https://doi.org/10.1080/00498254.2020.1729446
24. Bourqui A, Niang EHAB, Graz B, et al. Hypertension treatment with Combretum micranthum or Hibiscus sabdariffa, as decoction or tablet: a randomized clinical trial. Journal Human Hypertensi. 2021;35(9):800-808. doi:https://doi.org/10.1038/s41371-020-00415-1
25. Amos A, Khiatah B. Mechanisms of Action of Nutritionally Rich Hibiscus sabdariffa’s Therapeutic Uses in Major Common Chronic Diseases: A Literature Review. Journal of the American Nutrition Association. 2022;41(1):116-124. doi:https://doi.org/10.1080/07315724.2020.1848662
26. Nyam KL, Teh YN, Tan CP, Kamariah L. In vitro antioxidant activities of extract and oil from roselle (Hibiscus sabdariffa L.) seed against sunflower oil autoxidation. Malaysian Journal Nutrition. 2012;18(2):265-274.
27. Alsayed M.A. et al. Nutrients Aqueous Fraction from Hibiscus sabdariffa Relaxes Mesenteric Arteries of Normotensive and Hypertensive Rats through Calcium Current. Mdpi. 2020;12:1782.
28. Efosa JO, Omage K, Azeke MA. Drying temperature affects the hypolipidemic, antioxidant, and antihypertensive potential of Hibiscus sabdariffa calyx in rats induced with L-NAME. Toxicol Reports. 2023;11(August):177-188. doi:https://doi.org/10.1016/j.toxrep.2023.09.005
29. Nurfaradilla SA, Saputri FC, Harahap Y. Pharmacokinetic Herb-Drug Interaction between Hibiscus sabdariffa Calyces Aqueous Extract and Captopril in Rats. Evidence-based Complement Altern Med. 2020;2020. doi:https://doi.org/10.1155/2020/5013898
30. Al-Anbaki M, Nogueira RC, Cavin AL, et al. Treating uncontrolled hypertension with hibiscus sabdariffa when standard treatment is insufficient: Pilot intervention. Journal Altern Complement Med. 2019;25(12):1200-1205. doi:https://doi.org/10.1089/acm.2019.0220
31. Elkafrawy N, Younes K, Naguib A, et al. Antihypertensive efficacy and safety of a standardized herbal medicinal product of Hibiscus sabdariffa and Olea europaea extracts (NW Roselle): A phase-II, randomized, double-blind, captopril-controlled clinical trial. Phytotherapy Research. 2020;34(12):3379-3387. doi:https://doi.org/10.1002/ptr.6792
32. Salem MA, Ezzat SM, Ahmed KA, Alseekh S, Fernie AR, Essam RM. A Comparative Study of the Antihypertensive and Cardioprotective Potentials of Hot and Cold Aqueous Extracts of Hibiscus sabdariffa L. in Relation to Their Metabolic Profiles. Frontiers in Pharmacology. 2022;13(February):1-17. doi:https://doi.org/10.3389/fphar.2022.840478
33. Ramli ESM, Sukalingam K, Kamaruzzaman MA, Soelaiman IN, Pang KL, Chin KY. Direct and indirect effect of honey as a functional food against metabolic syndrome and its skeletal complications. Diabetes, Metab Syndr Obes. 2021;14:241-256. doi:https://doi.org/10.2147/DMSO.S291828
34. Natar Fitri Napitupulu1, Mastiur Napitupulu2 HS. The Effect of Giving Bee Honey on Blood Pressure in Elderly Patients with Hypertension. CHMK Nursing Scientific Journal. 2020;4(3):303-309.doi: https://doi.org/10.51933/health.v6i2.566
35. Imtara H, Al-Waili N, Bakour M, Al-Waili W, Lyoussi B. Evaluation of antioxidant, diuretic, and wound healing effect of Tulkarm honey and its effect on kidney function in rats. Veterinary World. 2018;11(10):1491-1499. doi:https://doi.org/10.14202/vetworld.2018.1491-1499
36. Hashim KN, Chin KY, Ahmad F. The mechanism of honey in reversing metabolic syndrome. Molecules. 2021;26(4). doi:https://doi.org/10.3390/molecules26040808
37. Musyayyadah SA, Darni J, Fatimah F. Effect of Honey Solution on Blood Pressure of Elderly Hypertension. Nutri-Science: Journal of Nutrition, Food and its Applications. 2019;3(2):83. doi:https://doi.org/10.21580/ns.2019.3.2.3425
38. Nasrullah PR, Sehar B, Safdar M, et al. Effect of Ajwa date pits powder (Phoenix dactylifera L.) on body composition, lipid profile and blood pressure in patients with hyperlipidemia: A randomized clinical trial. Avicenna J Phytomedicine. 2023;13(2):143-152. doi:https://doi.org/10.22038/AJP.2022.21316
39. Qasim N, Shahid M, Yousaf F, et al. Therapeutic Potential of Selected Varieties of Phoenix Dactylifera L. Against Microbial Biofilm and Free Radical Damage to DNA. Dose-Response. 2020;18(4):1-9. doi:https://doi.org/10.1177/1559325820962609
40. Alam MZ, Ramachandran T, Antony A, Hamed F, Ayyash M, Kamal-Eldin A. Melanin is a plenteous bioactive phenolic compound in date fruits (Phoenix dactylifera L.). Scientific Reports. 2022;12(1):1-12. doi:https://doi.org/10.1038/s41598-022-10546-9
41. Husaidah S, Ikhtiar M, Nurlinda A. The Effect of Giving KurmaAjwa (Phoenix dactylifera) on Changes in Blood Pressure of Hypertensive Pregnant Women. Health Journal. 2019;2(1):34-43. doi:https://doi.org/10.33096/woh.v2i1.580
42. Syafriati A, Ana P. The Effect of Giving Date Juice to Patients With High Blood Pressure at Pengarayaan Health Center. Tambusai Health Journal. 2024;5:2342-2350. doi:https://doi.org/10.31004/jkt.v5i1.26097
43. Pratiwi SW, Kurdanti W, Setyobroto I. The Effect of Giving Dates Infused Water on Changes in Potassium Levels in Students of Poltekkes Kemenkes Yogyakarta with Prehypertension. Medika Respati: Journal of Health Sciences. 2020;16(3):1-21. doi:https://doi.org/10.35842/mr.v16i3.520
44. Fitri Y, Rusmikawati R, Zulfah S, Nurbaiti N. Sodium and Potassium Intake as Causal Factors of Hypertension in the Elderly. Aceh Nutrrition Journal. 2018;3(2):158. doi:https://doi.org/10.30867/action.v3i2.117
45. Ekaningrum AY. Associations of sodium intake, fat, mental-emotional disorders, and lifestyle with hypertension in adults in Jakarta. Journal of Nutrition College. 2021;10(2):82-92. doi:https://doi.org/10.14710/jnc.v10i2.30435
46. Da Usfa M, Hasni D, Birman Y, Febrianto BY. Relationship between Potassium Intake and Hypertension in Ethnic Minangkabau Women. Nutrition Journal. 2023;12(2):52-63. doi:https://doi.org/10.26714/jg.12.2.2023.52-63
47. Utari S, Hamidah M, Wati DA, Dewi AP, Pratiwi AR. Relationship between Fat and Fiber Adequacy Levels with Hypertension in the Elderly of the Regional Technical Implementation Unit for Social Services for the Elderly Tresna Werdha Lampung in 2021. Aisyah Nutrition Journal. 2021;4(2):1-5.doi: https://doi.org/10.30604/jnf.v5i1.550