Ethanol Extracts of Coleus tuberosus Flesh and Peel as a Potential Source of Natural Antioxidant and Breast Cancer Prevention Agent

Mutiara Nugraheni^1*, Badraningsih Lastariwati¹, Windarwati²

¹Department of Culinary Art Education, Engineering Faculty, Yogyakarta State University, Indonesia
²The Center of Public Hospital, Indonesian Ministry of Health, Yogyakarta, Indonesia.

Corresponding Author Email: mutiara_nugraheni@uny.ac.id

This study examines the effects of ethanol extracts of Coleus tuberosus flesh and peel on T47D cancer cells. Antioxidant potential was evaluated through cellular antioxidant activity experiment, and anti-proliferation activity was evaluated using MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide). The effects on cell cycle were evaluated with a flow cytometry, while induction of apoptosis was evaluated based on morphological changes by staining with acridine orange and ethidium bromide. The results indicate that the peel extract had higher cellular antioxidant activity than the flesh extract. The IC50 of celullar antioxidant activity of the flesh and peel extracts were 287.13±10.35 g/ml and 217.86±12.96 g/ml, respectively. The peel extract also had higher anti-proliferative activity. The IC50 of anti-proliferative activity were 887.05±5.03 µg/ml (flesh extract) and 548.18±4.52 µg/ml (peel extract). The peel and flesh extracts can cause cell cycle arrest in the S phase and G2-M phase. The extracts induce apoptosis within T47D cancer cells, showing an orange color. Therefore, these extracts could be used as potential sources of natural antioxidants and breast cancer prevention agents.

Antioxidant; Anti-proliferation; Apoptosis; Cell cycle; Ethanol extract of Coleus tuberosus.

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