Molecular Detection of Vancomycin Resistance Genes in Staph aureus Isolates from Different Clinical Specimens

Hiba Jasim Hamza^1*, Mays Hadi Jebur¹, Jwan A. Ali² and Zuhair Mohammed Ali Jeddoa³

¹Department Basic and Medical Science, University of Babylon. Babble, Iraq.

²Department of Microbiology, University of Babylon, Babble, Iraq

³College of Pharmacy, Al-Zahraa University for Women, Iraq.

Corresponding Author E-mail: nur.hiba.jassim@uobabylon.edu.iq

The study screened the occurrence of VanA, VanB, Vanc genes in Staph aureus vancomycin-resistant isolates recovered in Hilla -hospitals. From various clinical samples, 160 isolates from patients between July and November of 2022 were included in the search. Among these samples are (65) burn samples, (55) urine samples, and (40) samples from Blood. 40 (or 25%) of the 160 isolates were Staph aureus. Characterization of the VanA, VanB, and VanC genes in Staph aureus isolates obtained from various specimens is the main goal of the work. The RESULTS of samples were obtained from a variety of clinical specimens, including 20 (40.625%) burn swabs, 10 (25%) urine samples, and 10 (34.375%) blood. Staph aureus was resistant to Cefepime (91.7%), Piperacillin (73.3%), and Ceftazidime (71.7%), although little relationship was detected with Aztreonam resistance (35%). 40 isolates were used for antibiotic sensitivity test (AST). Only 18.3% and 15% of isolates, respectively, were resistant to meropenem and imipenem. Fluoroquinolone resistance to lomefloxacin, ciprofloxacin, ofloxacin, and levofloxacin was 51.7%, 48.3%, 35%), and 30%, respectively. While the most prevalent vancomycin resistance genes were found in 40 isolates of S. aureus and were discovered by VanC 40 (100%), VanA 20 (100%), and VanB28 (70%), according to the current investigation. In Conclusion :The PCR-reaction method was discovered to be an easy-to-use tool for detecting staph aureus by pcr that can be regularly used for identifying staph aureus and detection the molecular resistance apparatuses to vancomycin in staph aureus by VanA, VanB, VanC), efflux systems plays To choose the best antibiotics, prevent the establishment of resistant strains, and create novel antimicrobial. medications, it is beneficial to research the particular resistance mechanisms vancomycin to staph aureus.

ESKAPE; MDR; Staph aureus; VanA; VanB; VanC; Vancomycin Resistance

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