Antibacterial Activity of Black Pepper Essential Oil Nanoemulsion Formulated by Emulsion Phase Inversion Method

Ly Thi Minh Hien^1,2,3* and Dong Thi Anh Dao^1,2

¹Division of Food Technology, Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), Ho Chi Minh City, Vietnam.

²Vietnam National University Ho Chi Minh City, Ho Chi Minh City, Vietnam.

³Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam.

Corresponding Author Email: ltmhien.sdh17@hcmut.edu.vn

Black pepper essential oil has been proved to inhibit the growth of microorganisms in many recent studies. However, free essential oils are often lipophilic and difficult to use in food products. The nanoemulsion has some advantages such as good dispersion, long-term stability, and transparency. In our study, the Emulsion Phase Inversion method was utilized to formulate black pepper essential oil nanoemulsion. After 6 months, the nanoemulsion retained the droplet size about 18 nm and there was a rise in polydispersity index from 0.087 to 0.608. Besides, concentrations of important components (α-pinene, β-pinene, D-limonene, 3-carene, and β-caryophyllene) in the BPEO phase of nanoemulsion were similar to pure essential oil. This study was also showed that Escherichia coli and Salmonella enterica were sensitive to black pepper essential oil nanoemulsion than free essential oil. Minimal Inhibitory Concentrations of nanoemulsion for E. coli and S. enterica (137 and 273 µg/mL, respectively) were higher than those of free essential oil (547 µg/mL). In addition, nanoemulsion inhibited these bacterial growth on pork samples. When utilizing nanoemulsion as a meat preservative, meat samples, which contained nanoemulsions, observed significantly lower aerobic microbial counts than control samples.

Antibacterial Activity; Black Pepper Essential Oil Nanoemulsion; Emulsion Phase Inversion; Meat Preservation

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