The Effect of Vitamin D Administration on Leptin, Adiponectin and mRNA MCP-1 Levels in Adipose Tissue of Obese Female Wistar Rats

Luh Putu Ratna Sundari^1*, Made Bakta², Nyoman Mantik Astawa³, Putu Gede Adiatmika¹, Gusti Kamasan Nyoman Arijana⁴, Ketut Tunas⁵

¹Physiology Department, Faculty of Medicine, Udayana University, Bali, Indonesia
²Internal Medicine Department, Faculty of Medicine-Sanglah Hospital, Udayana University, Bali, Indonesia
³Immunology Laboratory, Faculty of Veterinary, Udayana University, Bali Indonesia
⁴Histology Department, Faculty of Medicine, Udayana University, Bali, Indonesia
⁵Department of Public Health, Faculty of Health, Science and Technology, Dhyana Pura University, Bali, Indonesia

Corresponding Author Email: luhputu_ratnafk@unud.ac.id

In obesity, there is an accumulation of adipocytes which produces adipokine that are pro-inflammatory substance, such as leptin and MCP-1 and anti-inflammatory substance, such as adiponectin, while the bioavailability of vitamin D is decreased. This research aimed to study the effect of vitamin D administration on leptin, MCP-1, and adiponectin levels in adipose tissue rats with obesity. Vitamin D was administered to the obese model of 6-9 months old female Wistar rats. This experiment was a randomized control group design with a post-test group design only. Twenty-seven (27) female obese Wistar rats were included in this study. The animals were divided randomly into 3 groups: 9 rats were given 2400 IU vitamin D (group A), 9 rats were given 800 IU vitamin D (group B) and 9 rats were given a placebo as control (group C). The administration of Vitamin D was given once daily for 8 weeks. The visceral adipose tissue was taken to measure the level of leptin, adiponectin and mRNA MCP-1. Data among groups was analyzed by using one-way ANOVA and followed by LSD test, at a significance level of p <0.05. The lowest level of leptin (1059.15+135.20 pg/ml) and mRNA MCP-1 (2.36 + 0.75 fg/ml) and the highest adiponectin level (3.43 + 0.47 ng/ml) were found in group A. In conclusion, oral administration of vitamin D (2400 IU) decreased pro-inflammatory substances, such as leptin and mRNA MCP-1 and increased anti-inflammatory substances, such as adiponectin, in visceral adipose tissue of obese female Wistar rats.

Adiponectin; Leptin; mRNA MCP-1; Obesity; Vitamin D

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