The Effect of Nitrogen Fertilisation and Metabolic Regulators SNRK1, GCN2 on the Formation of Acrylamide in two Potato Varieties (Spunta and Lady Rosetta) Fried in Corn Oil

D. Argyropoulos¹ Ch. Psallida¹ and T. Varzakas^2*

¹Elgo Dimitra, Greece, Dept. Food Technology, Kalamata, Greece
²TEI Peloponnese, Dept. Food Technology, Kalamata, Greece

The effect of nitrogen fertilization and metabolic regulators SNRK 1, GCN 2 on the formation of acrylamide in two potato varieties (Spunta and Lady Rosetta) fried in corn oil was studied. For this study, the effect of cultivation treatments involving the combination of two levels of nitrogen during potato cultivation [treatment 1: N1=300mg/kg of soil, treatment 2: N2=600mg/kg of soil)] was analysed in relation to the mRNA expression of the genes SnRK1 and GCN2 and acrylamide formation. Results indicated that increased concentrations of nitrogen fertilization caused a reduction in mRNA expression of SnRK1 and GCN2 genes for both varieties. An increase in nitrogen fertilization caused a reduction in expression of SnRK1 gene and in reducing sugar formation; while mRNA expression for GCN2 decreased and total soluble protein and acrylamide formation increased. Increased expression of SnRK1 and GCN2 genes was observed at low fertilization (low nitrogen) accompanied with a reduction in acrylamide concentration in both varieties. On the contrary, while increased nitrogen resulted in lower levels of reducing sugars but higher protein levels, higher acrylamide levels were observed attributed to increased amino acid levels and specifically asparagine.

SNRK1; GCN; acrylamide; potato tubers; corn oil

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